YEPD (non-selection)
1% yeast extract
2% peptone
1.5% agar (if needed for plates)
After autoclaving add glucose to 2% by adding 100 ml of 20% solution to each liter media.
SYNTHETIC MEDIA (selective media)
0.67% yeast nitrogen base without amino acids (Difco) 0919 15 3
1.5% agar (if needed for plates). Also add 1 pellet of NaOH per litre of agar to avoid mushy plates.
After autoclaving add 100 ml of 20% glucose (or galatose) solution and 100 ml of 10X Dropout solution to each liter media.
10X DROPOUT SOLUTION
L-Isoleucine 300 mg/L
L-Valine 1500mg/L
L-Adenine hemisulfate salt 200 mg/L
L-Arginine HCl 200 mg/L
L-Lysine HCl 300 mg/L
L-Methionine 200 mg/L
L-Phenylalanine 500 mg/L
L-Threonine 2000mg/L
L-Tyrosine 300 mg/L
L-Uracil 200 mg/L
L-Histidine HCl monohydrate 200 mg/L* Sigma H9511
L-Leucine 1000mg/L* Sigma L1512
L-Tryptophan 200 mg/L* Sigma T0271
*The 10X Dropout solution does not contain Histidine, Leucine and Tryptophan. These components are used to make selective media to select for yeast transformants.
** A better option is to purchase selective dropout amino acid mix. CSM -His,-Leu,-Trp -- BIO 101 (Cat. 10427-100) and add either His, Leu or Trp as needed.