PCR reaction conditions:
1 X Amplitaq reaction buffer
2.0 mM Mg Cl2
0.2 mM dNTPs
0.4 uM primer F
0.4 uM primer R
0.625 Units Amplitaq / reaction
1.6 ng/ul Template DNA

94^oC denaturing, 5 minutes
Followed by 35 cycles of
94^oC denaturing, 1 minute
58^oC annealing, 1 minute
72^oC extension, 1.5 minutes
72^oC final extension, 5 minutes