Targeted mutagenesis with the maize transposable element Ds in barley
is made possible by a tendency for the element to reinsert near the original
site of excision. The aim of this project is the generation of populations
of individual barley lines, each with an independently transposed, mapped
Ds element, such that each region of the barley genome (bin) is represented by at least one line containing a Ds insertion.
To exploit localized transposition for gene tagging, transgenic barley
stocks with insertions located near genes of interest will be candidates
for secondary activation of the elements to permit efficient tagging based
on short-range transposition of Ds.
January 2006: To date 137 lines with transposed Ds-bar elements have been identified in barley. Of those 137, flanking sequences have been determined for ~80 lines and currently 35 have been mapped (19 map locations were published (see below); Cooper et al., 2004.
Publications:
Cooper LD, Marquez-Cedillo L, Singh J, Sturbaum AK, Zhang S, Edwards V, Johnson K, Kleinhofs A, Rangel S, Carollo V, Bregitzer P, Lemaux PG, Hayes PM (2004) Mapping Ds insertions in barley using a sequence based approach. Molecular Genetics and Genomics 272: 181-193. (Pubmed Link)
Singh J, Zhang S, Lemaux PG, Bregitzer P, Sturbaum AK, Edwards V, Hayes PM, Cooper LD, Marquez-Cedillo L, Carollo V (2005) Maize Transposable Elements and Barley: A New Population for Genetic Research. Barley Genetics Newsletter 35: 1-2
Zhang S, Chen C, Lei L, Meng L, Singh J, Jiang N, Deng X-W, He Z-H, Lemaux PG (2005) Evolutionary Expansion, Gene Structure, and Expression of the Rice Wall-Associated Kinase Gene Family. Plant Physiology 139:1107-1124. (Pubmed Link)
Singh J, Zhang S, Chen C, Cooper L, Bregitzer P, Sturbaum AK, Hayes PM, Lemaux PG (2006) High-frequency Reactivation of Maize Ds Favors Saturation Mutagenesis in Barley. Plant Molecular Biology online first: DOI 10.1007/s11103-006-9067-1.
Meng L, Ziv M, Lemaux PG (2006) Nature of Stress and Transgene Locus Influences Transgene Expression Stability in Barley. Plant Molecular Biology 62: 15-28 (Springer Link)
Bregitzer, P., L. Cooper, P. Hayes, P.G. Lemaux, J. Singh, and A. Sturbaum. (2006) Viability and bar expression are negatively correlated in Oregon Wolfe Barley dominant hybrids. Plant Biotechnology Journal: accepted.
Singh, J., H. Randhawa, P.G. Lemaux, and K.S. Gill. (2006) The Ac/Ds transpositions in barley occur preferentially in the gene-rich regions. Molecular Genetics and Genomics: submitted.
The Interactive Oregon Wolfe Barley Maps (IMap) provide
an interactive resource to view barley bin maps with links to information
on the individual TNP lines, naked-eye phenotypes (NEPs) and provides links
to the GrainGenes database from all other loci.
Lemaux Lab, University of California, Berkeley
Peggy G. Lemaux (lemauxpg@nature.Berkeley.edu)
Jaswinder Singh
Shibo Zhang
Hayes Lab, Oregon State University
Patrick Hayes (patrick.m.hayes@oregonstate.edu)
Lol Cooper
Luis Marquez-Cedillo
Bregitzer Lab, National Small Grains Germplasm Research Facility, USDA-ARS, Aberdeen, Idaho
Phil Bregitzer (pbregit@uidaho.edu)
Anne Sturbaum
GrainGenes Project, USDA-ARS-WRRC, Albany, CA
Victoria Carollo (vcarollo@pw.usda.gov)
Instructions for Requesting TNP lines and Ac Transposase lines
