Nomenclature of BAC clones and libraries
TAACSP3BFhA_0001A23 is a BAC clone with the following specifications:
TAA defines the genus/species. Three characters are used since there was
concern two would not be enough to clearly define all possible cases.
[See Registry of Species Abbreviations.]
CSP defines the cultivar. Three characters since we're concerned two won't
be enough in future, and to handle cultivars that already have a standard
3-letter designation. (Some have more, but those few would be shortened
for the BAC names. Some have less, like Chinese Spring, so they need to be
lengthened.) e.g. CSP = Chinese Spring, CNN = Cheyenne.
[See Registry of Germplasm Abbreviations.]
The third set of 3 characters defines the chromosomal source of the DNA.
Examples are:
3BF = full 3B chromosome
3BL = long arm of 3B
3BS = short arm of 3B
ALL = total genome library
146 = the library of the combined 1D, 4D, and 6D
chromosomes. This is an unusual case and not
likely to have many other such examples. If
necessary, unique 3-character identifiers will
be created.
hA defines a restriction enzyme used to make the library, in this case
HindIII, with this being the first instance of a HindIII library
for this genus, species, cultivar. If a second HindIII library were made
from the same source, it would be hB. Lowercase first character to make
more readable.
The "_" separates the library name from the specific clone
identification within that library. Its main function is to improve
readability, instead of the continuous long stream of characters which
the eye will tend to blur. Also, BAC libraries and clones within a
library are quite different conceptually and in usage.
0001A23 identifies plate number and well position within the plate:
Plate no. 1, position A23 of the indicated library. Note that four digits
must be used for the plate number.
Thus, TAACSP3BFhA_0001A23 is clone 0001A23 from the first HindIII
library of the full 3B chromosome of Chinese Spring, Triticum aestivum
ssp. aestivum.
In publications, any shorter library names can be used as appropriate for
the libraries in that publication, and referenced to the full name in the
Abbreviations footnote. Clones may be referred to by clone name only (ex:
0001A23) within a publication and only after being defined - unless fuller
names are necessary for clarification.
The registry of BAC library names is at
http://wheat.pw.usda.gov/GG2/BAClibrary.shtml.
For information about existing Triticeae BAC libraries see
Jorge Dubcovsky's database.