The Plan
Derive unigene set from CUGI NRI ESTs (13 libraries - 63,602 sequences)
Pick ESTs & genomic sequences representing genes of agronomic importance (eg., induced by biotic and abiotic stress)
Mask non-unique sequences by comparison to homology database & repetitive elements
Design 8,400 60-mers for in situ synthesis on glass slides (Agilent technologies)