cDNA Libraries

This page last updated June 01, 2001.

cDNA Libraries from Triticum aestivum cv. Chinese Spring
cDNA Libraries from other Triticum aestivum cultivars
cDNA Libraries from other Triticeae species
ITEC
Proposed Libraries

cDNA Libraries from Triticum aestivum cv. Chinese Spring

See the expanded version of this table here (provided by T. Close)

For detailed library description click on library name.

Albany Library # Tissue Developmental Stage Treatment Sequencing Status Date Library Received ESTs produced % singleton candidates % library unique ESTs Tissue Source PI Library Source Lab

TA006E1X shoot seedling etiolated Done 7/11/99 1839 70.3 24.6 T Close, UCR T Close, UCR

TA006E2N shoot seedling etiolated Done 4/17/00 1702 35.5 4.9 T Close, UCR H Nguyen, TTU

TA006E3N shoot seedling etiolated In progress 03/07/01 - - - T Close, UCR H Nguyen, TTU

TA005E1X whole seedling dehydration Done 7/11/99 796 87.8 26.1 T Close, UCR T Close, UCR

TA005E2S whole seedling dehydration Done 7/19/00 921 67.9 - T Close, UCR H Nguyen, TTU

TA008E1X root seedling etiolated Done 9/27/99 4017 69.2 24.5 T Close, UCR T Close, UCR

TA008E2N root seedling etiolated On hold 4/11/00 - - - T Close, UCR H Nguyen, TTU

TA008E3N root seedling etiolated Done 4/17/00 4310 62.1 20.9 T Close, UCR H Nguyen, TTU

TA007E1X whole seedling cold Done 9/27/99 943 85.1 25.3 T Close, UCR T Close, UCR

TA007E2S whole seedling cold On hold 7/19/00 - - - T Close, UCR H Nguyen, TTU

TA007E3S whole seedling cold In progress 03/07/01 - - - T Close, UCR H Nguyen, TTU

TA019E1X spike pre-anthesis white to yellow anther Done 3/20/00 10544 63.9 30.9 T Close, UCR T Close, UCR

TA018E1X spike post-anthesis 5 to 15 DAP Done 3/20/00 2860 69.8 24.4 T Close, UCR T Close, UCR

TA017E1X spike post-anthesis 20 to 45 DAP Done 3/20/00 1076 51.0 14.1 T Close, UCR T Close, UCR

TA016E1X crown seedling vernalized Done 3/20/00 2286 80.4 30.1 T Close, UCR T Close, UCR

TA015E1X whole 14 day old seedlings 2 hour heat Done 3/20/00 824 80.9 30.6 H Nguyen, TTU T Close, UCR

TA031E1X flag leaf anthesis heat Done 6/27/00 977 81.5 33.3 H Nguyen, TTU T Close, UCR

TA025E1X green leaf full tillering stage normal On hold 5/24/00 - - - H Nguyen, TTU T Close, UCR

TA026E1X spikes pre-anthesis unstressed On hold 5/24/00 - - - K Gill, UNL T Close, UCR

TA032E1X spikes post-anthesis heat 5-20DAP Done 6/27/00 1013 79.9 33.8 H Nguyen, TTU T Close, UCR

TA036E1X leaf full tillering stage drought Done 8/8/00 641 84.2 30.4 H Nguyen, TTU T Close, UCR

TA037E1X sheath seedling salt Done 8/8/00 964 70.7 24.8 J Dvorak, UCD T Close, UCR

TA038E1X crown seedling salt Done 8/8/00 943 86.7 28.8 T Close, UCR T Close, UCR

TA042E1X seed post-anthesis 3 to 6 DAP In progress 11/06/00 692 80.3 64.5 JP Gustafson, UMC T Close, UCR

TA047E1X root tip seedling unstressed In progress 03/02/01 - - - JP Gustafson, UMC T Close, UCR

TA056E1X root full tillering stage drought stressed In progress 05/01/01 - - - H Nguyen, TTU T Close, UCR

TA057E1X root tip seedling aluminum stressed In progress 05/01/01 - - - JP Gustafson, UMC T Close, UCR

cDNA Libraries from other Triticum aestivum cultivars

For detailed library information click on library name.

Albany Library # Cultivar Tissue Developmental Stage Treatment Sequencing Status Date Library Received ESTs Produced % singleton candidates % library unique ESTs Tissue Source PI Library source Lab

TA001E1X Cheyenne endosperm 5-30 days post anthesis control In progress 9/27/95 2729 54.0 19.7 O Anderson, USDA/ARS O Anderson, USDA/ARS

TA001E1S Cheyenne endosperm 5-30 days post anthesis subtracted In progress - 274 87.9 27.4 O Anderson, USDA/ARS O Anderson, USDA/ARS

TA001E2N Cheyenne endosperm 5-30 days post anthesis normalized In progress 03/02/01 - - - O Anderson, USDA/ARS H Nguyen, TTU

TA009XXX Sumai3 spikes mature plant challenged with Fusarium graminearum In progress 2/16/00 4750 75.3 33.7 G Meuhlbauer, UMN G Meuhlbauer, UMN

- Sumai3 spikes mature plant challenged with Fusarium graminearum - - 727 - - John Fellers, Bikram Gill, KSU John Fellers, Bikram Gill, KSU

TA027E1X TAM W101 leaf full tillering drought stress Done 5/24/00 911 79.7 30.7 H Nguyen, TTU T Close, UCR

TA012XXX Brevor embryos mature, dormant seeds seeds imbibed in 20 microM ABA for 12 hrs at 30C Done - 2211 79.5 35.1 Kay Walker-Simmons, WSU Kay Walker-Simmons, WSU

TA049E1X Brevor embryos mature, dormant seeds plants grown under conditions favoring seed dormancy In progress 03/02/01 555 76.0 26.7 Kay Walker-Simmons, WSU T Close, UCR

TA048E1X BH1146 root tip 4-day old plants Aluminum stressed In progress 03/02/01 312 73.7 23.7 JP Gustafson, UMC T Close, UCR

cDNA Libraries from other Triticeae species

For detailed library information click on library name.

Albany Library # Species Cultivar or Accession Tissue Developmental Stage Treatment Sequencing Status Date Library Received ESTs Produced % singleton candidates % library unique ESTs Tissue Source PI Library Source Lab

TM011XXX Triticum monococcum DV92 shoot apex of tillers 5-week old plants vegetative apex Done - 2857 79.1 37.8 J Dubcovsky, UCD J Dubcovsky, UCD

TM043E1X Triticum monococcum DV92 shoot apex of tillers 7-week old plants early reproductive apex Done 12/01/01 2647 71.7 30.8 J Dubcovsky, UCD J Dubcovsky, UCD

TM046E1X Triticum monococcum G3116 shoot apex 1-month old plants vernalized In progress 02/28/01 - - - J Dubcovsky, UCD J Dubcovsky, UCD

SC013XXX Secale cereale Blanco root tips young roots control Done 2/17/00 778 90.4 49.0 JP Gustafson, UMC JP Gustafson, UMC

SC010XXX Secale cereale Blanco root tips young roots stressed for Aluminum Done 2/17/00 1199 84.2 43.6 JP Gustafson, UMC JP Gustafson, UMC

SC024E1X Secale cereale Blanco anther mature plants control Done 5/24/00 4640 56.7 26.2 JP Gustafson, UMC T Close, UCR

TT039E1X Triticum turgidum Langdon-16 whole plant mature plant normal Done 8/8/00 1194 74.7 25.5 S Kianian, NDSU T Close, UCR

AS040E1X Aegilops speltoides F2s from 2-12-4-8-1-1-1-(1) x PI36909-12-811-(1) anther premeiotic stage normal Done 8/8/00 2466 70.5 29.0 J Dvorak, UCD T Close, UCR

ITEC

For an expanded version of this table, click here

Triticeae species of origin Genotype or cultivar or accession of origin Tissue Developmental stage Condition* Vector Size of library (est. no. of clones) Contact (if library is available) ITEC contact if source of ESTs

Libraries from which sequences will be contributed to ITEC

Aegilops speltoides . anthers immature; at meiosis normal conditions . . . Jan Dvorak

. . . . . . . . .

Lophopyrum elongatum Accession D root seedling salt-stressed with 250 mM NaCl for 6 hours under hydroponic conditions. PBluescript SK-, converted from original cDNA library in Lambda zap about 500,000 P. Gulick P. Gulick

. . . . . . . . .

Triticum aestivum Atlas (Al tolerant) root 8 day old grown in vermiculite with 0.6 mM CaCl2 in water pYES2 (Invitrogen - yeast expression plasmid vector) 8.7x10 ^6 . Marilyn Warburton

Triticum aestivum Bobwhite endosperm mature seed nonstressed and insect-infected (Russian wheat aphid) . . Anna-Maria Botha-Oberholster Anna-Maria Botha-Oberholster

Triticum aestivum Catoctin (USDA-ARS, Beltsville MD) leaf four fully developed leaves after seed germination normal greenhouse conditions pSport-P Primary library 1.12x10^7 cfu/ml J.-J. Lin J.-J. Lin

Triticum aestivum Chinese Spring anther early meiosis . . . . Peter Langridge

Triticum aestivum Chinese Spring root . . . . Dave Lonsdale Paul Bailey

Triticum aestivum Chinese Spring scutelum callus undifferentiated . . . Dave Lonsdale Paul Bailey

Triticum aestivum Chinese Spring spikelet immature, developing . . . Graham Moore Paul Bailey

Triticum aestivum Chinese Spring spikes immature . pT-Adv 100 . Bikram Gill

Triticum aestivum Gamtoos-R (1RL.1BL translocation) leaf & stem 12- to 15-day-old seedlings infected with Russian Wheat Aphid pCMV Sport 6.0 not complete, est. at 3 million . Nora Lapitan,
Dennis Knudson

Triticum aestivum Germplasm line P29 leaf & stem 9-day-old seedlings unstressed Lambda Zap Express 1 million . Joseph M. Anderson

Triticum aestivum Mustang leaf 10-day-old seedlings heat stress Lambda Zap II 2 million Henry Nguyen Henry Nguyen

Triticum aestivum Norin 26 spikelet Feeke's scale 6 field grown . 782,000 fpu Yasunari Ogihara Yasunari Ogihara

Triticum aestivum Novosibirskaya 67, red grain isoline pericarp/testa 3:1 mix, respectively of 10+20 days post anthesis . Lambda Zap II 2 million Paul Bailey, John Flintham Paul Bailey

Triticum aestivum Odeon root seedling unstressed pUC18 819 J.M. Jacquemin J.M. Jacquemin

Triticum aestivum Palmiet leaf 4-leaf stage nonstressed and insect-infected (Russian wheat aphid) . . Anna-Maria Botha-Oberholster Anna-Maria Botha-Oberholster

Triticum aestivum Soleil endosperm 1:1 mix of 10+20 days post anthesis . Lambda Zap II 2 million Kay Denyer Paul Bailey

Triticum aestivum Sumai #3 spikes at anthesis challenged with head blight fungus pGemT 200 . Bikram Gill

Triticum aestivum TAM 101 root 10-day-old seedlings water deficiency stress Lambda Zap II 400,000 Henry Nguyen Henry Nguyen

Triticum aestivum Thatcher Lr1 leaf young leaf 24 h after challenge with LR-AVR1 Lambda Zap 1 million Sylvie Cloutier Sylvie Cloutier

Triticum aestivum Tugela endosperm mature seed nonstressed and insect-infected (Russian wheat aphid) . . Anna-Maria Botha-Oberholster Anna-Maria Botha-Oberholster

Triticum aestivum Wyuna endosperm mid-development . Lambda Zap . . Rudi Appels

Triticum aestivum . embryo . . . . . Mike Holdsworth

Triticum aestivum . endosperm . . . . . Mike Holdsworth

Triticum aestivum . leaf seedling challenged with an avirulent isolate of wheat yellow rust Lambda Zap Express . Lesley Boyd, Rob Koebner Paul Bailey

Triticum turgidum ssp. durum Siliana root 3-day-old seedling drought stress pSPORT1 (BRL) about 50,000 before amplification Philippe Joudrier Philippe Joudrier

*condition- environmental stress, disease challenge, etc.

Proposed Libraries to be Included in the EST Sequencing Project

Please post comments and further cDNA library suggestions to the Project Bulletin Board.

Henry Nguyen

A hexaploid wheat (cv TAM 101, hard red winter wheat) of special interest subjected to drought stress. See bulletin board
Salt stressed root tissue. See bulletin board
Alumintum stressed roots from aluminum tolerant wheat cultivar (BH1146) if wheat rather than rye is used. See bulletin board
Chinese Spring developing seeds under heat stress (whole spikes collected at 5,10,15,20,25,30,35,40,45 DAA after the plants are exposed to heat stress at these development time points for 5 days).
Chinese Spring developing seeds under drought stress (whole spikes collected at 7,14,21,28,35,42 DAA after the plants are exposed to a gradual drought stress to each specific time point).

Mark Sorrells and Kay Walker-Simmons

A hexaploid wheat of special interest subjected to heat stress. See bulletin board

Kay Walker-Simmons
Note: At least some of the following cDNA libraries are suggested to be from a dormant variety such as Clark's Cream.

bulletin board

Optimum dormancy development - Grow wheat under well-watered, cool greenhouse conditions. Collect seeds at milky dough through hard dough. Enrich by cutting embryonic axes and pool.
Imbibed dormant (germination suppressed, even under favorable conditions for germination) - Imbibe dormant seeds in water. Collect seeds at 0-12 h. I would suggest enriching by cutting embryonic axes off. I would suggest imbibing the seeds and collecting embryos over a number of time points. My preference is to imbibe the seeds at a warmer temperature (25C) to optimize dormancy expression.
Germination (early germination genes) - Imbibe afterripened, nondormant seeds and then collect seeds at 0-12 h. Embryonic axes and aleurone could be dissected and pooled.
ABA - responsive - Imbibe embryos or whole seeds (dormant) in 25 uM ABA. Collect embryos at 0-12 h.
GA - responsive - Imbibe embryos and aleurone (nondormant) in 25 uM GA. Collect aleurone layers at 0-12 h.

Perry Gustafson

Rye anthers. See bulletin board
Rye aluminum stressed roots. See bulletin board
Root libraries from the spring hexaploid wheat 'BH1146', the most aluminum tolerant spring wheat. Tissues will collected from stressed and non-stressed roots. See bulletin board

Shahryar Kianian

Langdon Durum Wheat. See bulletin board

Jorge Dubcovsky

Shoot apexes from T. monococcum. See bulletin board.
Subtraction library from T. monococcum. Pooled RNAs from different point times of differentiation of the shoot apex for at least two genotypes (one winter G3116 and one spring G2528) subtracted by the DV92 apex cDNA library. See bulletin board.

Olin Anderson

Tissue culture libraries. See bulletin board.
Chinese Spring developing seeds under heat stress (whole spikes collected at 5,10,15,20,25,30,35,40,45 DAA after the plants are exposed to heat stress at these development time points for 5 days).
Chinese Spring developing seeds under drought stress (whole spikes collected at 7,14,21,28,35,42 DAA after the plants are exposed to a gradual drought stress to each specific time point).

Albany Library #	Tissue	Developmental Stage	Treatment	Sequencing Status	Date Library Received	ESTs produced	% singleton candidates	% library unique ESTs	Tissue Source PI	Library Source Lab
TA006E1X	shoot	seedling	etiolated	Done	7/11/99	1839	70.3	24.6	T Close, UCR	T Close, UCR
TA006E2N	shoot	seedling	etiolated	Done	4/17/00	1702	35.5	4.9	T Close, UCR	H Nguyen, TTU
TA006E3N	shoot	seedling	etiolated	In progress	03/07/01	-	-	-	T Close, UCR	H Nguyen, TTU
TA005E1X	whole	seedling	dehydration	Done	7/11/99	796	87.8	26.1	T Close, UCR	T Close, UCR
TA005E2S	whole	seedling	dehydration	Done	7/19/00	921	67.9	-	T Close, UCR	H Nguyen, TTU
TA008E1X	root	seedling	etiolated	Done	9/27/99	4017	69.2	24.5	T Close, UCR	T Close, UCR
TA008E2N	root	seedling	etiolated	On hold	4/11/00	-	-	-	T Close, UCR	H Nguyen, TTU
TA008E3N	root	seedling	etiolated	Done	4/17/00	4310	62.1	20.9	T Close, UCR	H Nguyen, TTU
TA007E1X	whole	seedling	cold	Done	9/27/99	943	85.1	25.3	T Close, UCR	T Close, UCR
TA007E2S	whole	seedling	cold	On hold	7/19/00	-	-	-	T Close, UCR	H Nguyen, TTU
TA007E3S	whole	seedling	cold	In progress	03/07/01	-	-	-	T Close, UCR	H Nguyen, TTU
TA019E1X	spike	pre-anthesis	white to yellow anther	Done	3/20/00	10544	63.9	30.9	T Close, UCR	T Close, UCR
TA018E1X	spike	post-anthesis	5 to 15 DAP	Done	3/20/00	2860	69.8	24.4	T Close, UCR	T Close, UCR
TA017E1X	spike	post-anthesis	20 to 45 DAP	Done	3/20/00	1076	51.0	14.1	T Close, UCR	T Close, UCR
TA016E1X	crown	seedling	vernalized	Done	3/20/00	2286	80.4	30.1	T Close, UCR	T Close, UCR
TA015E1X	whole	14 day old seedlings	2 hour heat	Done	3/20/00	824	80.9	30.6	H Nguyen, TTU	T Close, UCR
TA031E1X	flag leaf	anthesis	heat	Done	6/27/00	977	81.5	33.3	H Nguyen, TTU	T Close, UCR
TA025E1X	green leaf	full tillering stage	normal	On hold	5/24/00	-	-	-	H Nguyen, TTU	T Close, UCR
TA026E1X	spikes	pre-anthesis	unstressed	On hold	5/24/00	-	-	-	K Gill, UNL	T Close, UCR
TA032E1X	spikes	post-anthesis	heat 5-20DAP	Done	6/27/00	1013	79.9	33.8	H Nguyen, TTU	T Close, UCR
TA036E1X	leaf	full tillering stage	drought	Done	8/8/00	641	84.2	30.4	H Nguyen, TTU	T Close, UCR
TA037E1X	sheath	seedling	salt	Done	8/8/00	964	70.7	24.8	J Dvorak, UCD	T Close, UCR
TA038E1X	crown	seedling	salt	Done	8/8/00	943	86.7	28.8	T Close, UCR	T Close, UCR
TA042E1X	seed	post-anthesis	3 to 6 DAP	In progress	11/06/00	692	80.3	64.5	JP Gustafson, UMC	T Close, UCR
TA047E1X	root tip	seedling	unstressed	In progress	03/02/01	-	-	-	JP Gustafson, UMC	T Close, UCR
TA056E1X	root	full tillering stage	drought stressed	In progress	05/01/01	-	-	-	H Nguyen, TTU	T Close, UCR
TA057E1X	root tip	seedling	aluminum stressed	In progress	05/01/01	-	-	-	JP Gustafson, UMC	T Close, UCR

Albany Library #	Cultivar	Tissue	Developmental Stage	Treatment	Sequencing Status	Date Library Received	ESTs Produced	% singleton candidates	% library unique ESTs	Tissue Source PI	Library source Lab
TA001E1X	Cheyenne	endosperm	5-30 days post anthesis	control	In progress	9/27/95	2729	54.0	19.7	O Anderson, USDA/ARS	O Anderson, USDA/ARS
TA001E1S	Cheyenne	endosperm	5-30 days post anthesis	subtracted	In progress	-	274	87.9	27.4	O Anderson, USDA/ARS	O Anderson, USDA/ARS
TA001E2N	Cheyenne	endosperm	5-30 days post anthesis	normalized	In progress	03/02/01	-	-	-	O Anderson, USDA/ARS	H Nguyen, TTU
TA009XXX	Sumai3	spikes	mature plant	challenged with Fusarium graminearum	In progress	2/16/00	4750	75.3	33.7	G Meuhlbauer, UMN	G Meuhlbauer, UMN
-	Sumai3	spikes	mature plant	challenged with Fusarium graminearum	-	-	727	-	-	John Fellers, Bikram Gill, KSU	John Fellers, Bikram Gill, KSU
TA027E1X	TAM W101	leaf	full tillering	drought stress	Done	5/24/00	911	79.7	30.7	H Nguyen, TTU	T Close, UCR
TA012XXX	Brevor	embryos	mature, dormant seeds	seeds imbibed in 20 microM ABA for 12 hrs at 30C	Done	-	2211	79.5	35.1	Kay Walker-Simmons, WSU	Kay Walker-Simmons, WSU
TA049E1X	Brevor	embryos	mature, dormant seeds	plants grown under conditions favoring seed dormancy	In progress	03/02/01	555	76.0	26.7	Kay Walker-Simmons, WSU	T Close, UCR
TA048E1X	BH1146	root tip	4-day old plants	Aluminum stressed	In progress	03/02/01	312	73.7	23.7	JP Gustafson, UMC	T Close, UCR

Triticeae species of origin	Genotype or cultivar or accession of origin	Tissue	Developmental stage	Condition*	Vector	Size of library (est. no. of clones)	Contact (if library is available)	ITEC contact if source of ESTs
Libraries from which sequences will be contributed to ITEC
Aegilops speltoides	.	anthers	immature; at meiosis	normal conditions	.	.	.	Jan Dvorak
.	.	.	.	.	.	.	.	.
Lophopyrum elongatum	Accession D	root	seedling	salt-stressed with 250 mM NaCl for 6 hours under hydroponic conditions.	PBluescript SK-, converted from original cDNA library in Lambda zap	about 500,000	P. Gulick	P. Gulick
.	.	.	.	.	.	.	.	.
Triticum aestivum	Atlas (Al tolerant)	root	8 day old	grown in vermiculite with 0.6 mM CaCl2 in water	pYES2 (Invitrogen - yeast expression plasmid vector)	8.7x10 ^6	.	Marilyn Warburton
Triticum aestivum	Bobwhite	endosperm	mature seed	nonstressed and insect-infected (Russian wheat aphid)	.	.	Anna-Maria Botha-Oberholster	Anna-Maria Botha-Oberholster
Triticum aestivum	Catoctin (USDA-ARS, Beltsville MD)	leaf	four fully developed leaves after seed germination	normal greenhouse conditions	pSport-P	Primary library 1.12x10^7 cfu/ml	J.-J. Lin	J.-J. Lin
Triticum aestivum	Chinese Spring	anther	early meiosis	.	.	.	.	Peter Langridge
Triticum aestivum	Chinese Spring	root	.	.	.	.	Dave Lonsdale	Paul Bailey
Triticum aestivum	Chinese Spring	scutelum callus	undifferentiated	.	.	.	Dave Lonsdale	Paul Bailey
Triticum aestivum	Chinese Spring	spikelet	immature, developing	.	.	.	Graham Moore	Paul Bailey
Triticum aestivum	Chinese Spring	spikes	immature	.	pT-Adv	100	.	Bikram Gill
Triticum aestivum	Gamtoos-R (1RL.1BL translocation)	leaf & stem	12- to 15-day-old seedlings	infected with Russian Wheat Aphid	pCMV Sport 6.0	not complete, est. at 3 million	.	Nora Lapitan, Dennis Knudson
Triticum aestivum	Germplasm line P29	leaf & stem	9-day-old seedlings	unstressed	Lambda Zap Express	1 million	.	Joseph M. Anderson
Triticum aestivum	Mustang	leaf	10-day-old seedlings	heat stress	Lambda Zap II	2 million	Henry Nguyen	Henry Nguyen
Triticum aestivum	Norin 26	spikelet	Feeke's scale 6	field grown	.	782,000 fpu	Yasunari Ogihara	Yasunari Ogihara
Triticum aestivum	Novosibirskaya 67, red grain isoline	pericarp/testa	3:1 mix, respectively of 10+20 days post anthesis	.	Lambda Zap II	2 million	Paul Bailey, John Flintham	Paul Bailey
Triticum aestivum	Odeon	root	seedling	unstressed	pUC18	819	J.M. Jacquemin	J.M. Jacquemin
Triticum aestivum	Palmiet	leaf	4-leaf stage	nonstressed and insect-infected (Russian wheat aphid)	.	.	Anna-Maria Botha-Oberholster	Anna-Maria Botha-Oberholster
Triticum aestivum	Soleil	endosperm	1:1 mix of 10+20 days post anthesis	.	Lambda Zap II	2 million	Kay Denyer	Paul Bailey
Triticum aestivum	Sumai #3	spikes	at anthesis	challenged with head blight fungus	pGemT	200	.	Bikram Gill
Triticum aestivum	TAM 101	root	10-day-old seedlings	water deficiency stress	Lambda Zap II	400,000	Henry Nguyen	Henry Nguyen
Triticum aestivum	Thatcher Lr1	leaf	young leaf	24 h after challenge with LR-AVR1	Lambda Zap	1 million	Sylvie Cloutier	Sylvie Cloutier
Triticum aestivum	Tugela	endosperm	mature seed	nonstressed and insect-infected (Russian wheat aphid)	.	.	Anna-Maria Botha-Oberholster	Anna-Maria Botha-Oberholster
Triticum aestivum	Wyuna	endosperm	mid-development	.	Lambda Zap	.	.	Rudi Appels
Triticum aestivum	.	embryo	.	.	.	.	.	Mike Holdsworth
Triticum aestivum	.	endosperm	.	.	.	.	.	Mike Holdsworth
Triticum aestivum	.	leaf	seedling	challenged with an avirulent isolate of wheat yellow rust	Lambda Zap Express	.	Lesley Boyd, Rob Koebner	Paul Bailey
Triticum turgidum ssp. durum	Siliana	root	3-day-old seedling	drought stress	pSPORT1 (BRL)	about 50,000 before amplification	Philippe Joudrier	Philippe Joudrier