| June 8 status | ||||||||||
| No. of clones received since May 1 | No. blots on hand (all five gels) | No. of clones on hand still to map | No. of mapped clones for which data are submitted | No. of mapped clones for which data have not yet been submitted | No. of mapped clones for which autorad image files have been submitted | No. of unmappable clones (+reason) | Mapping throughput (clones per week) | Name of principal worker in charge of mapping | Comment | |
| J Anderson | 36, 138 total | 12 sets of 5, 40 by Aug 31 | 17 | 75 | 20 | 75 | 26: 14 high background; 12 elim by prev screening | 12 due to supply, capacity for 20 to 40 | J Gonzalez, full-time postdoc, with 2 ugrads | No problems with inserts. |
| J Dubcovsky | 85, 207 total | 60 sets | 82 | 49 | 0 | 28, 21 more to be submitted by June 15 | 72: most, 50, were from 1st plate produced under different selection conditions; remap attempts will be made on some. | 20 is capacity, but with insert problems, average is lower | Anura Ratanasiri, postdoc with assistance of 2 ugrads | Replacement of DNAs has meant new blots are necessary, high cost in time and money for troubleshooting. Producing own inserts should improve mapability. |
| J Dvorak | 36, 151 total | 40 | 36 | 80 | 0 | 80 | 35, all high copy | 20 is possible, but with recent insert problems, average since May 1 has been 3.3 | Eduard Akhunov, postdoc with assistance of 2 ugrads. | Continuing problem with inserts means need to re-amplify before hybridizing; would therefore rather get plasmids. |
| B Gill | 96, 337 total | 52 sets of five | 42 | 225 | 53 | 196 | 20: 15 high copy, 5 weak signal | 18 to 20 | ||
| K Gill | 0, 110 total | 50 sets of all five available, to have 64 sets by end of June | 85 | 0 | 20 to be submittedbyn June 11 | 0 | 6, some smear pattern, so bands not clear | 10, target is 20 | Temp postdoc Lijia Ljiah, currently recruiting full-time postdoc | Of the 110 clones, got only 25 as inserts and rest as plasmids; purifying inserts from plasmids is delay; prefer getting inserts. |
| P Gustafson | 0, 73 total | 11 sets of four blots with some blank lanes, 15 sets of five blots | 36 | 5 | 10, to submit by 6/13 | 8 | 11 highly repetitive | 10 | Miftahudin, grad student, with assistance of ugrads. | Had power failures, lost hyb oven, had to re-hyb. |
| S Kianian | 0, 124 total | 50 sets of all five | 4, more efficient to have a set of 15, so waiting | 35, had to rescore first set | 59, to be submitted by 6/15 | 35 | 26: 6 empty wells, 4 high background, 16 didn't work, need again | 30 to 40 | K Hossain, half-time postdoc -- V Kalavacharla, half-time postodc -- J Hegstad, 20% technician | Need about 50 clones per week, better is two full 96-well plates (i.e. total of 196) per month. |
| N Lapitan | 0, 62 total | 15 sets of 5 blots (4 blots in 7 sets have gone bad, leaving only 8 good sets); 11 sets of 4 blots (testing for quality; they appear to have lower DNA conc. than the 8 good sets) | 45 | 0, last report indicated that 30 to 40 were expected to be submitted by this date. | 17 | 0 | 4; 2 had strong background smear and weak bands; 1 was a repeated sequence with no bands; 1 had no missing bands in all the lines | 8 (this is the no. of good complete sets we have on hand); target is 30. | Junhua Peng, postdoc, with part-time assistance of postdoc Shifang, technician H. Zadeh, and ugrad D Arellano. | Reorganized work assignments, current bottleneck is no. of blots, goal is to be up to 30 per week by July 15. |
| H Nguyen | 24, 24 total | 1 set of five, 10 sets by June 15 | 24 | 0 | 0 | 0 | none known yet | 20 by June 25 | D Zhang, full-time postdoc, with 2 ugrads | Had DNA digestion difficulties so blot preparation delayed. |
| M Sorrells | 36, 167 total | 60 sets of all five | 22 | 52 | 76 | 52 | 17: high background and high copy | Max 40, usually 30 | C Bermudez, technician, whose last month is July, will need to hire and retrain in Aug/Sept | Had to re-map a lot of the clones because the deletion line filters had to be made again after the workshop. |