Complementary DNA libraries from wheat or closely related species will be produced and normalized to maximize the access to all genes in the wheat genomes. Approximately 80,000 of these cDNA clones will be single-pass 5' sequenced thereby producing a large database of expressed sequence tags (ESTs). Identification of singleton gene motifs from these ESTs, and those generated by international collaborators, will be carried out by 20,000 3' single-pass sequencing. Nucleotide sequence comparison of these ESTs will be used to arrive at a minimum of 10,000 EST singletons, representing approximately half of all the gene motifs in the wheat genomes. All chromosomal loci complementary to these singletons in the wheat genomes will be identified by their Southern hybridization with a unique mapping population of chromosome deletion stocks. Rice, maize and other grass genome ESTs homologous to the wheat singletons will be identified by nucleotide sequence match searches. Additional rice and maize ESTs of known chromosomal position in those genomes and not matching wheat ESTs will be mapped in wheat. These two approaches will produce detailed comparative maps between wheat and other grass genomes. The pool of wheat EST singletons will be used in gene-function studies focusing on the reproductive phase of the wheat plant. The database generated by these objectives will provide means to study specific questions about the structure, putative function, chromosomal location, and evolution of the expressed component of the wheat genomes.
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NSF abstract- The official project abstract on the NSF webpage
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