Protocols for Wheat SNP Discovery
- IDs of line for the production of A, B and D genome PCR amplicons and lines for the first round of SNP screening
- PCR with genomic DNA
- TA subcloning using Promega pGEM-T Easy Vector System (Promega, Cat#: A1360) Transformation of electrocompetent cells by electroporation
- Amplifigation of subcloned Langdon amplicons
- Sequencing reaction with Big Dye Terminator v 3.1 (ABI)
- Purification of sequencing products
- Preparation of amplicons for shipping to Albany for sequencing