Protocols
				Protocols for wheat SNP discovery (Updated on Oct. 12, 2004 IDs of line for the production of A, B and D genome PCR amplicons and  lines for the first round of SNP screening (Updated on Nov. 22, 2004) PCR with genomic DNA TA subcloning using Promega pGEM-T Easy Vector System (Promega, Cat#: A1360) Transformation of electrocompetent cells by electroporation Amplifigation of subcloned Langdon amplicons Sequencing reaction with Big Dye Terminator v 3.1 (ABI) Purification of sequencing products Preparation of amplicons for shipping to Albany for sequencing