Sequence ID: DB080G17_R1.ab1

Motif: (TAAA)3 Motif length: 4 SSR: TAAATAAATAAA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 724 21 54.89 42.86 4.00 3.00 AACCAGTTCAGTTCACTCTGA 162 921 921 5.00 3.00   REVERSE 885 21 55.12 42.86 2.00 1.00 AGAGTTTGTGTAGCAGGTTGA Primer view: 1 TCGACTCTGAGGATCCGATGGCTAACATCGACTGGTTTCTAACTAACAGCAGGTGACTCA 61 TCAATAGGCAAACCGACATATATATATAACTAGCCAACAACCATATGACATTGTACCACA 121 TAGGAAGTTAAACAAGAACCTAGCTAGCAAGCGAGGTATATATCGATCCTGTCACTACAT 181 ACGTGGTTCAACTTTCCAGTGATAAATTGATACTACCAAGCAAGAACGGTACGTTTGACG 241 CAGCTGATGAAGCAAGTAATACATTTCCTATGCATGCTCGTGGGCACGAGAGGCGTCAAG 301 AATGCTCTCGGAAAACTAATACTCCCAAGAAGACGGATGGTGATGCTCTCTTCAATGGCC 361 TCATCTACGGCAGTCCTTAACCAGCTTCTTTATCCAGCAGGAGTTCGTCGTGTTCCGATG 421 ATCTTCCTGCCCTTCTGGTTCTGCTCTCTTTTTCTTTTTTACAGAGAGAAAGCCAAGAAG 481 ACGAAGCGCAATCAAACCAGCGCATCGTTAGCAGAGAAAGCCATGAAAATTTTGTGTGCC 541 AGGGCAATTCACAAGCTTAGTGGTGATGGATGGATTAACGCAGGCTTCAATGTTTCTCTA 601 GTGTGTACTAATGTTTTACTTTAGTCATCACGGACTAAAACTTAACCAAACAAAGACAAA 661 GGAGAACAAAACAAAATTAGCAGTAATTAGTGAGCGTCTACTGCTAGTTTCGTTTCATAG 721 AACAACCAGTTCAGTTCACTCTGAGTCGTGCACAAGTTTGGATACATCGAGATGATTAGC       >>>>>>>>>>>>>>>>>>>>>                                    781 ACATTTCTTTCAAGGCCGGTCCTGAGATTTCGGGCCCCCAGGCGAAACTTAAACTTGAGC 841 CCTTAAATAAATAAAACAACCGTTTCAACCTGCTACACAAACTCTAATTTCGTCTGCTTC                           <<<<<<<<<<<<<<<<<<<<<                901 ATGTGAGCGATGTTTTGGCAC Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0