Sequence ID: DB080F05_R1.ab1

Motif: (TCTGG)3 Motif length: 5 SSR: TCTGGTCTGGTCTGG SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 663 22 55.00 40.91 3.00 0.00 GTCAGGAACATAGAAGTGGTTT 142 919 919 5.00 3.00   REVERSE 804 20 55.14 40.00 5.00 3.00 CCTTGAAATTGTTTCTACGC Primer view: 1 AGGATCCGACGATGAGTGAGGTTCGTCGTTATGTTGTGGGTCATGTGCTTACATTCCAAC 61 ATGCTCTCACCTTGGAGTAGTTGTGGCCCTTATGTTCTACCCTTTTCCGCTGCGGTATTT 121 TGAAACATTGTTTTATGATGTTGAAACACTATTTATTTTATGCCGGCCGAAGAGGTCATG 181 CGAGGTTGTAAGTACTATTTAAATCCTGGATGATGCGATGTAATAAAGTACTTTTGACCT 241 TTGTTTCTGTATATTTCATCATGAAACTATGTGTGCTAGTGAGTCGATCCAGGGACTAGC 301 ACAGTAAGCACAGAAATCAAACCCTGTAAGGGGGGGAATCGCTTCAACAACACACTATGA 361 ATGCTTGATGAGCACATGGATAATTTTCACACTTGTTAAATAGATCCATGAATGTTGAAG 421 AACGTAGAAAGCTATTTCCCAACTTAAACAAAATACATATGGGTGAACTCCATCCGTAGT 481 AAAAAAACATCAAGAAAACCTAATATAATGCATGCAATGAAAATTCATTGGCAAGGATTA 541 ACTGTAGCACGTTCAGTAACATGTAATACCATGCAAAAAAGCCACCATATCCCTTTTCCT 601 TTAAGCTAATATTCAAGTAAAAATTATTTCAAGGATACTACTATTGTGGAACATTTTATG 661 AAGTCAGGAACATAGAAGTGGTTTTCAATAAATTGCATAATTGTGTATAGTCTTATTCTG      >>>>>>>>>>>>>>>>>>>>>>                                    721 GTCTGGTCTGGTCTTATTACATATTTTATGTGCTCCAAGATTTTCATAAAAGTTGCGTTT 781 TGCAGCGTAGAAACAATTTCAAGGCTTTTAGAAAGCAAACATTTAAGAACTAAACTAATT       <<<<<<<<<<<<<<<<<<<<                                     841 TTTACATAGGGAAACATTAATATTTTTTCACTTTAAAAAATATATCGGATTCCTACATTT 901 ACATGTCTCACTTCTTCTG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0