Sequence ID: DB085C05_R1.ab1

Motif: (GCC)5 Motif length: 3 SSR: GCCGCCGCCGCCGCC SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 655 20 55.37 40.00 4.00 1.00 CTGATCTGAAACTTGGCATT 149 913 913 5.00 2.00   REVERSE 803 21 54.51 42.86 4.00 1.00 TCTGGATCATGGATGTCTACT Primer view: 1 TCGACTCTAGAGGATCCATCCAGTTCTTGAATTGGCCTGATCTTTCATCTTTCGTTGTCC 61 TTGAAATTAAAGGAGAATTTTGTGTAACTGTCAAATCTTTTGGTCTCTGTCTAACATTCG 121 CTTAATTTTTGCCAACGTTTTCTTTAACTCGGATTTGAAGTACACCTGACGTTTTTTCCT 181 GGGTACGAAATCAGGGCATGGCTTGCTGAATCATCCAATGTTGCATATTCTCTGGTGACT 241 GCTGAGTAATACTGCCTCGATCTTGACCAATTTAATTAGGTCAGTCAAGATATAACACAG 301 CAACCTAGACTTACGCATAATCTCGATCCGGCCGGCTGTTCCAATGCAAACTCAAGATTT 361 TTAGGATCTCTGATGAAAGTATATGCTATTCGCCAATTCGGTAGCGAGAGCAGAGATACT 421 GGTCCTACGCCAAGTAGCCGCACGAAGCTTACTCCTGCTTACTAAAGAGAATCCCGAGGT 481 GGGAACCAGTCGCCCGATCAGTAGCAATTTACATAGTACTGGAGTACTACTGATGCCGGA 541 GATGCCCAACTAATGAGAGCTTTTTCCATAATAAAATGAGGAGTACCAAGCGGCAATTGA 601 TTTAGAAGGATGTTTTAGGTATTTTCTCTCATCTAACGGCTAATTATTTACTAACTGATC                                                          >>>>> 661 TGAAACTTGGCATTCATGCCGCCGCCGCCGCCTGAGATCGCGCCTGGCCGCCGGCATCTC   >>>>>>>>>>>>>>>                                              721 GTTACTTTCCCGACCAACGATGACGTGTATTTGGGCTCCCTCGCCACCACTGCGACCATG 781 GGAGTAGACATCCATGATCCAGACATCTATTCAGATTGAAACGCATGCAGGAAAGATGGA     <<<<<<<<<<<<<<<<<<<<<                                      841 GCTCTGCCATGAACTTGATCAAATCAATCACGTTTAGATCAGATGCTTGTAGCCGCAGCC 901 ACTGGGATCCTTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0