Sequence ID: DB085M03_R1.ab1

Motif: (GTC)4 Motif length: 3 SSR: GTCGTCGTCGTC SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 100 19 55.23 47.37 8.00 2.00 TAATGGTCACGTGAGATGG 134 913 913 6.00 2.00   REVERSE 233 20 55.02 40.00 4.00 3.00 TAATTTGACCTGACGACACA Primer view: 1 TGAGGATCCGGAGCACAAGCGCGACCGGTGCCTGGGTCTCCACGGTTTCCCGGGCTTTGT 61 TTACCGCACCGAAGGCGTTGAATGTCTGCTGCTCCCATTTAATGGTCACGTGAGATGGAA                                           >>>>>>>>>>>>>>>>>>>  121 GCCAGCTCGCGATGCCCGCGTTGGCTGTCGTCGTCGTCGCTGGACGCTCTGGATCGCGAT 181 CGGTCAGCTGCGACTGCGGGCGGAGCAGCGAGATGTGTCGTCAGGTCAAATTAATCGCGC                                    <<<<<<<<<<<<<<<<<<<<        241 TGGATTTGTGGATTGGATTTTGATCCTTGCCTGTTGGCTCGCTGAGGCTGGCGCGGTGTT 301 CCCGAGGCTTCTTTCACTGGACTGTTGTGGCACCTTGTGAAGAGTACTGGCATGTGGTGC 361 TCTATTTTTTTTGGAGGAGGTGCGAACTGGGTTTTGTCCGGAAAGGTTTAGCTCCTGTTG 421 CGTTGACCGACTGGCCAAACCATGCTCGACCACTCAGCCACTGAGCCACCGTTAGGTTTT 481 GCCTCACGTCGCTGGAGCACAACGGTTCAGGCGTTCGTCCTACTCATTCACAAATACTCC 541 CTGCCTCCGTCCAATATGACTTTCTATTACGTTACATGTATGTATCTAGACGTCTTCTAA 601 GCATATATACATTCATATTTATGCACATTTGAGTCATTTAATATGAGACGAAAAAAGTAA 661 GTTTGGTATACGTAAAAAAAATAGTTTTAACGAATTTTGTTGAAAAGATTAACAACACAT 721 ATAACATCAGATCAGTGTATTGTGAAACGGATATGACGATTCTAATTTAATGTCATAAAT 781 GTTTTTGATGGAAATAATGTCACAAATGTTGATACGTTTTCCAATAAAGTCGGTCATATT 841 TTAATATTTTTGATTTGGGACAAAATCGAAAAGTACACTTGTTTGTGGACGGAGAGAGTA 901 TGACAAATTTGAT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0