Sequence ID: DB088L13_F1.ab1

Motif: (ATT)4 Motif length: 3 SSR: ATTATTATTATT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 581 21 54.98 42.86 6.00 2.00 ATGTCTCTTAATTCCGAGGTC 145 910 910 5.00 0.00   REVERSE 725 20 54.67 40.00 2.00 0.00 ACGATTGAGAAAGTGGTTGT Primer view: 1 CCTACTACCCGTTCACTTTTCAAATCTCCTACTATTTAAATTATTCCTCGAATTTTGAAT 61 AAGAGATTTGAGAATTGAACCGAAGGAGGCCTTTCGCGGGTCCTGTTTGCGTCCCTACAT 121 TCCGTGACGGTCCGACGAACCTCCCATGTACTAGTTTCTAGCTAGGTTAATTTACAGATC 181 GATGGATGCCCAGCTCACCATATGGTATTTGCGCTAACCCTTGGAGCCCTCCCATAATCT 241 AGGTCAAAAATAATTGCACCATCTAGTGGTAGGCTACGACTCTTTATTTGGTCATTTGTT 301 GATGCAGGCCATTTTCATTAGTTCCATTGTTGCGTGCAGTCTTAGAGCAGAGACTGAAAA 361 TAGTGAACATTTCACTAGCTACCAGTGAATAAGAATTGGTCACAAGCAAGGTTTTAACTA 421 TCGGATTCCGTTTTACCTTCCCGGGAACCGGGATTTGCGGTAACAAGAAAATCCGAACAA 481 TTTGTTAGACATCATATTCTATCAAATTTAAACATATTTTTTCCTTTTGGAAATGATTAT 541 GCATTACACATTCATAAATTCTGTCTACAACCTGTAGTCCATGTCTCTTAATTCCGAGGT                                            >>>>>>>>>>>>>>>>>>> 601 CACAGGTTCATTGTGGGTTAAGTTCAATATTTTTTTAATAAAACAAGCATTATTATTATT   >>                                                           661 TTGTGGGGAAGAAAATCCTGATCGGACGACAATGTCAATCCAGAAACAACCACTTTCTCA                                                <<<<<<<<<<<<<<< 721 ATCGTCCGAGCAATACGCCGAGCTAGTTTCACATATACAAGCATCGTTCTCTTTTTCTCC   <<<<<                                                        781 TGTCTGCAACCTTACCACACAAGGATTCAGAACCCAGTGCGCGGTTGTGCAATTGTTTAA 841 TTGTCAGCTTACTTGTAAGTGAAGAAGTGGCTAGCCAGCTTACTAGTGGTGCAGCTAATC 901 ATGAGACCCA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0