Sequence ID: DB086J03_R1.ab1

Motif: (TGTG)3 Motif length: 4 SSR: TGTGTGTGTGTG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 532 21 55.44 42.86 6.00 2.00 CCATTGTCTAGTGCTAATGGA 157 910 910 5.00 0.00   REVERSE 688 21 54.70 42.86 6.00 2.00 GTTGAGCTAACAAGCTGAAAG Primer view: 1 TCTAGAGGACCCGAGCGAGATATCGGGGACGCCTCCATATCCGTAACGGGACAGGGCTTC 61 TCCTGGAGAGCGTTTTTGAGCCCTTAACCTCCAAATATAGCTATTGGGGGCCTGATGGGG 121 CTTCTCCTGGAGTTGCTCTAATTCCACGTTATCCTCTTCCTTTCCCATTCCACCGCATTT 181 TTTTTTAGGGGAAGCCTTTGTTCGGTTGTTCCCCGCTAAGCCTGGCGAACGACGGCCGGG 241 CCGGGTACTAGCTGGCGATGGGCCAGCACGGAACGGGTGGCACAAAATGAAATCGTGCCG 301 TGTCAGTTTGGCCCACGGGCCTGATTTAGGAAAAAGGCCCGGCACACTGAACTGAGGTGG 361 TTATGGTTTTGACCACACAATCTTCTGGCTTTGGCATCCCATGCGATTGCGACTCCGGGC 421 AACTGGAGAGACGGCGGCTACTTTTCATCTGACTTCGTCCTCCGCTCGCCTAATCCATCC 481 TGGCTCCGTTGTCCGCTCGCCCAATCGCCCACTCCATCTCAGTCGCGGTCTCCATTGTCT                                                       >>>>>>>> 541 AGTGCTAATGGATAAACCGCTTGTGTGTGTGTGCGTGCCCGCAGTGTGCTAGCGATCTGG   >>>>>>>>>>>>>                                                601 CATGTAATTCTTAGGAGCGCTCGAATGCTTAAGAGGTTAACGAACTGAGTCGAACAAGAG 661 TTTTAGGCTTTCAGCTTGTTAGCTCAACGAGCATGAGCTTAATGAGTCAAGCATCTCGTG          <<<<<<<<<<<<<<<<<<<<<                                 721 ACGGCGAGGCAAAGATGTTTCAGGCCAGGCTGCCCTTGCCAGTTGGCAACGTAAATCTAT 781 ATTGCACTATCTGTTTTTATTTTATTTTTGACGACCTACTAAACAAAACCTCTGCTCATT 841 GAAAAATTGGAAATGGATCCTGCTTCACGTACGATCTTTTGCATCCGATTTTCATCAGGC 901 GGATCTATCA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0