Sequence ID: DB070A03_R1.ab1

Motif: (GCG)4 Motif length: 3 SSR: GCGGCGGCGGCG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 176 20 55.35 50.00 4.00 3.00 AAGCTACGGAGGAGAAACTC 165 909 909 4.00 0.00   REVERSE 340 20 55.51 40.00 4.00 2.00 CAATCCTGTCTCGAAGAAAA Primer view: 1 TCGACTCTGAGGATCCAGAAATCTTTTATAATCCATCGAACAATTAGGCACAAGCGCAAC 61 GAGCAGAGACATGCTGACTCTCGAACTAAACAGGAGCAGCAACAAATGGTATGTGTACAA 121 ACCGGAGTAAGCGCCGAGCTCGAGGGGGGAGAGGCGCATCTTGGCGGCGTGCTCGAAGCT                                                           >>>> 181 ACGGAGGAGAAACTCGAGCTGCCGCGCCGGCGGGAGTGCGGCCGACAGGAGGACTCCACC   >>>>>>>>>>>>>>>>                                             241 GCCCGCGGGTTCACCTGCAGCGGCGGCGGCGTTGATTTGGTCCTGTTCGGCGAGTGGGCG 301 ATGCTTCTTCTGCTTGCGCGTTTTCTTCGAGACAGGATTGGTTGATTTCACGGCGAGGGT                       <<<<<<<<<<<<<<<<<<<<                     361 ATTGGCGAGCATCTTCCGCTTGCCGGCGGTGGCGGCCTGCGTACGCGGCAACATTTTCAA 421 GCTACGGGGTATTGGCCTTTGGGCTTTTCACTGTCTAGGGTTTTGCTCGCTTTCAATGAG 481 GTCGGGTCGGATATGTATCCATGTTCGTTCGTGATTGTTTGGTATTCTATTTCACCTTTT 541 GCTGTAAAAGTTTATACATTTGAGAAGCAGGTCCGCGGTGCTTCTTGCAGCTTCTGGAGA 601 GAAACGCCTTCCGACGTGCTTCTCAACAACAAATGGTATGTGTACAAACCGGAGTTAGAA 661 GCACCAAAAGCTGAAACAAAAATTCAAACAAATAAGGCCACACCCTGCCTAAAGAATATA 721 ACTCTCAATCCAAAAGGGACCTATTTCTCGTCCTCACAACGCGCCACCTCAGCGAGTGCC 781 CATGTATTTTGATTTTTCGCAACAGTGCGAGTTTTTTGGGGTGGCCCGCTGTGAGTGGCC 841 TTGCGCAGGAATAGGCCTAGACGAAAAGATCGAAGTTCGACAATCTTATTGAACGCTCGC 901 TAACTCGGT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0