Sequence ID: DB083J20_F1.ab1

Motif: (CAG)5 Motif length: 3 SSR: CAGCAGCAGCAGCAG SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 532 18 53.85 55.56 4.00 1.00 GACCTCTTCAACGTCCAG 170 908 908 5.00 1.00   REVERSE 701 21 54.88 42.86 3.00 2.00 GTGGACAGACACATAAAAAGC Primer view: 1 TCACCGGCATGCCTTCTGACATGATTAATTTACCATGCTTACTTTTGGAGGCATATATAC 61 GTGGCAAGCATCCTTATATATACGTGGCATTGCCTAGCCGACACGTGACAAAGGCACGGC 121 CACGTGTCTGTCGATAACCGGCGTGGCAGGACGCATGCAAATTAAAAGTGGCCACGTCAC 181 CGAGCAACGTGTCGCCCCCAGCGCAAACAATGCATATCCTCCGACCTCTCCACACACACA 241 CAGCAGGGAACAGATCATATATACACCCTGCTGCAGTGTAGTGCAGCCGAGCATCAGCAG 301 AGCATATATACTCGTACAAGCAAGCAAGCAAAGTCAAGTATAATGGCGAGCGATAGCAGG 361 AGATCGCCGATGGAAGGGCAAGTTCCGGGGTACGGCAAGACGGTGGAGAGGGAGAAGAAG 421 ATGGAGCACGGGCGGGCGCAGGCCCGGCTAAGCGACGACGACATGCTCAGGACGGGCTAC 481 GTCGGCGAGACGCCGCTCGAAGGCGGCAAGATCGCCGACTCCGAACCCGTCGACCTCTTC                                                       >>>>>>>> 541 AACGTCCAGCCTCAGCAGCAGCAGCAGGCAATCCGAGGGGAAGAAGAAGATGAAGGGGAT   >>>>>>>>>>                                                   601 GAGAAGGAGCCGTCGGCGCCGAGAGGAGTGCAGCCGGCCAAGCAGGGGATGCACGGCGGC 661 CGTCAGCTAGGCCGCCAGTAGCTTTTTATGTGTCTGTCCACCGCCGTTGTATGGTACCAC                       <<<<<<<<<<<<<<<<<<<<<                    721 TAGCTAGCTAGTACTACTCGTGCTGTAATAAAGACCACAGGCCGCCGCCGACTACACCGC 781 CGGCCGGGCGGACAGCCTACGTCCTGCCTGAACTGAGCTCCATGTCGATCTCAGCTGAAC 841 CATCGTCAAACTATTCAGACTGTGGATCTGGTTCCCAAGTTTTACATGTTCCCGTTTTTG 901 TCAAATTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0