Sequence ID: DB083J20_F1.ab1

Motif: (CACA)3 Motif length: 4 SSR: CACACACACACA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 163 18 55.47 50.00 8.00 3.00 TAAAAGTGGCCACGTCAC 147 908 908 3.00 0.00   REVERSE 309 21 54.75 42.86 6.00 2.00 ATATATGCTCTGCTGATGCTC Primer view: 1 TCACCGGCATGCCTTCTGACATGATTAATTTACCATGCTTACTTTTGGAGGCATATATAC 61 GTGGCAAGCATCCTTATATATACGTGGCATTGCCTAGCCGACACGTGACAAAGGCACGGC 121 CACGTGTCTGTCGATAACCGGCGTGGCAGGACGCATGCAAATTAAAAGTGGCCACGTCAC                                              >>>>>>>>>>>>>>>>>> 181 CGAGCAACGTGTCGCCCCCAGCGCAAACAATGCATATCCTCCGACCTCTCCACACACACA 241 CAGCAGGGAACAGATCATATATACACCCTGCTGCAGTGTAGTGCAGCCGAGCATCAGCAG                                                   <<<<<<<<<<<< 301 AGCATATATACTCGTACAAGCAAGCAAGCAAAGTCAAGTATAATGGCGAGCGATAGCAGG   <<<<<<<<<                                                    361 AGATCGCCGATGGAAGGGCAAGTTCCGGGGTACGGCAAGACGGTGGAGAGGGAGAAGAAG 421 ATGGAGCACGGGCGGGCGCAGGCCCGGCTAAGCGACGACGACATGCTCAGGACGGGCTAC 481 GTCGGCGAGACGCCGCTCGAAGGCGGCAAGATCGCCGACTCCGAACCCGTCGACCTCTTC 541 AACGTCCAGCCTCAGCAGCAGCAGCAGGCAATCCGAGGGGAAGAAGAAGATGAAGGGGAT 601 GAGAAGGAGCCGTCGGCGCCGAGAGGAGTGCAGCCGGCCAAGCAGGGGATGCACGGCGGC 661 CGTCAGCTAGGCCGCCAGTAGCTTTTTATGTGTCTGTCCACCGCCGTTGTATGGTACCAC 721 TAGCTAGCTAGTACTACTCGTGCTGTAATAAAGACCACAGGCCGCCGCCGACTACACCGC 781 CGGCCGGGCGGACAGCCTACGTCCTGCCTGAACTGAGCTCCATGTCGATCTCAGCTGAAC 841 CATCGTCAAACTATTCAGACTGTGGATCTGGTTCCCAAGTTTTACATGTTCCCGTTTTTG 901 TCAAATTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

---

Primer3Batch v1.0