Sequence ID: DB083J20_F1.ab1

Motif: (CAAG)3 Motif length: 4 SSR: CAAGCAAGCAAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 203 19 54.04 42.11 6.00 0.00 GCAAACAATGCATATCCTC 149 908 908 4.00 0.00   REVERSE 351 21 56.22 42.86 4.00 0.00 GCTCGCCATTATACTTGACTT Primer view: 1 TCACCGGCATGCCTTCTGACATGATTAATTTACCATGCTTACTTTTGGAGGCATATATAC 61 GTGGCAAGCATCCTTATATATACGTGGCATTGCCTAGCCGACACGTGACAAAGGCACGGC 121 CACGTGTCTGTCGATAACCGGCGTGGCAGGACGCATGCAAATTAAAAGTGGCCACGTCAC 181 CGAGCAACGTGTCGCCCCCAGCGCAAACAATGCATATCCTCCGACCTCTCCACACACACA                          >>>>>>>>>>>>>>>>>>>                   241 CAGCAGGGAACAGATCATATATACACCCTGCTGCAGTGTAGTGCAGCCGAGCATCAGCAG 301 AGCATATATACTCGTACAAGCAAGCAAGCAAAGTCAAGTATAATGGCGAGCGATAGCAGG                                 <<<<<<<<<<<<<<<<<<<<<          361 AGATCGCCGATGGAAGGGCAAGTTCCGGGGTACGGCAAGACGGTGGAGAGGGAGAAGAAG 421 ATGGAGCACGGGCGGGCGCAGGCCCGGCTAAGCGACGACGACATGCTCAGGACGGGCTAC 481 GTCGGCGAGACGCCGCTCGAAGGCGGCAAGATCGCCGACTCCGAACCCGTCGACCTCTTC 541 AACGTCCAGCCTCAGCAGCAGCAGCAGGCAATCCGAGGGGAAGAAGAAGATGAAGGGGAT 601 GAGAAGGAGCCGTCGGCGCCGAGAGGAGTGCAGCCGGCCAAGCAGGGGATGCACGGCGGC 661 CGTCAGCTAGGCCGCCAGTAGCTTTTTATGTGTCTGTCCACCGCCGTTGTATGGTACCAC 721 TAGCTAGCTAGTACTACTCGTGCTGTAATAAAGACCACAGGCCGCCGCCGACTACACCGC 781 CGGCCGGGCGGACAGCCTACGTCCTGCCTGAACTGAGCTCCATGTCGATCTCAGCTGAAC 841 CATCGTCAAACTATTCAGACTGTGGATCTGGTTCCCAAGTTTTACATGTTCCCGTTTTTG 901 TCAAATTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0