Sequence ID: DB092D16_F1.ab1

Motif: (CTAG)3 Motif length: 4 SSR: CTAGCTAGCTAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 85 21 55.88 42.86 5.00 1.00 CTCGACTGCACTTTAAGACAA 163 947 947 4.00 1.00   REVERSE 247 21 55.47 42.86 3.00 2.00 GCTGTTCACTGTTGAGGATTA Primer view: 1 GGTATTCGCGGTTTTAAGATAACTTTGCACACAAAATGCTGTGTCCATCCCGTATGCAGA 61 GGCTGAGGGTTTTTTCGTGCATCGCTCGACTGCACTTTAAGACAATATAATTAATGCATC                            >>>>>>>>>>>>>>>>>>>>>               121 AATTATTGAAAAAAATTTGCCAACCTAGCTAGCTAGCTAATTCATTAATTATACTCCACT 181 AATTAGCAGGTCAAGTACAGTAGCCCGATCTCTTCGATCACCCCATTAATCCTCAACAGT                                                 <<<<<<<<<<<<<< 241 GAACAGCTTTAAATCGCCTGTTAATTCGTCACGTCTTTGCATTCATCTCTGCTTTCTTTC   <<<<<<<                                                      301 AATCTTTCATTCTTTACATCTTAGCAGGTGAGAACCGCGCGGGAACAACTATCCACTAAC 361 TTGCAATTAATTATAACGACGTTAATTAATCTGCGCCACACACTGATAAAGCTAGCTAGT 421 AGCTTGCAACACATGAAATGGATACTAAAAATAGCTATTAAAAGTAGCAGACCTATAGAG 481 ATCGTATATATGCACTCCCAGCTAGGACGTCCACTCGTCAAGATTAGCTTCACAGAATTG 541 ACTTCGAGACTTGGCTAGCTAGCTAGTAATATCGGTGAAGTATATATTCAGCTAATTTGA 601 CAGAGCCTTGCAGGTGAATTGTAGGCATGTCGATCACCTTAATTGATGGACAGCTACAAA 661 TCCAAGCAACTGTTTGGGTCTCAACCCAAGCAAATTGAGTGACAAGACACGCCACACGTC 721 GCTAGCTAGCAGCTAGCTTAGGGTAAGCTACAGTTTGTTGCATCACCAGCTGCTAGCTAG 781 GATATGTCTTCCAGCTTTCGTTTTCAAACTTTAAATTGTGAACATTTCCCTATTAATCAG 841 CCTCTCTCTATGCCGTGTGTCGACCAGCTGATCCTTCCCAGCCATGAGATCTGAGCTCAT 901 CGCTCACACCACTTTAATTTCCAACTGTGCATGAGGCCTCTGCTGTA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0