Sequence ID: DB083J20_F1.ab1

Motif: (CTAG)3 Motif length: 4 SSR: CTAGCTAGCTAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 681 21 54.88 42.86 3.00 2.00 GCTTTTTATGTGTCTGTCCAC 180 908 908 3.00 2.00   REVERSE 860 21 54.80 42.86 4.00 2.00 GTCTGAATAGTTTGACGATGG Primer view: 1 TCACCGGCATGCCTTCTGACATGATTAATTTACCATGCTTACTTTTGGAGGCATATATAC 61 GTGGCAAGCATCCTTATATATACGTGGCATTGCCTAGCCGACACGTGACAAAGGCACGGC 121 CACGTGTCTGTCGATAACCGGCGTGGCAGGACGCATGCAAATTAAAAGTGGCCACGTCAC 181 CGAGCAACGTGTCGCCCCCAGCGCAAACAATGCATATCCTCCGACCTCTCCACACACACA 241 CAGCAGGGAACAGATCATATATACACCCTGCTGCAGTGTAGTGCAGCCGAGCATCAGCAG 301 AGCATATATACTCGTACAAGCAAGCAAGCAAAGTCAAGTATAATGGCGAGCGATAGCAGG 361 AGATCGCCGATGGAAGGGCAAGTTCCGGGGTACGGCAAGACGGTGGAGAGGGAGAAGAAG 421 ATGGAGCACGGGCGGGCGCAGGCCCGGCTAAGCGACGACGACATGCTCAGGACGGGCTAC 481 GTCGGCGAGACGCCGCTCGAAGGCGGCAAGATCGCCGACTCCGAACCCGTCGACCTCTTC 541 AACGTCCAGCCTCAGCAGCAGCAGCAGGCAATCCGAGGGGAAGAAGAAGATGAAGGGGAT 601 GAGAAGGAGCCGTCGGCGCCGAGAGGAGTGCAGCCGGCCAAGCAGGGGATGCACGGCGGC 661 CGTCAGCTAGGCCGCCAGTAGCTTTTTATGTGTCTGTCCACCGCCGTTGTATGGTACCAC                        >>>>>>>>>>>>>>>>>>>>>                   721 TAGCTAGCTAGTACTACTCGTGCTGTAATAAAGACCACAGGCCGCCGCCGACTACACCGC 781 CGGCCGGGCGGACAGCCTACGTCCTGCCTGAACTGAGCTCCATGTCGATCTCAGCTGAAC                                                              < 841 CATCGTCAAACTATTCAGACTGTGGATCTGGTTCCCAAGTTTTACATGTTCCCGTTTTTG   <<<<<<<<<<<<<<<<<<<<                                         901 TCAAATTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0