Sequence ID: DB091N01_R1.ab1

Motif: (ACCA)3 Motif length: 4 SSR: ACCAACCAACCA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 638 21 54.82 42.86 3.00 3.00 ATGAGGAGGTTGGTAAGATTC 161 908 908 4.00 0.00   REVERSE 798 21 54.74 42.86 3.00 0.00 GAGGTTCTTTTTCTCCAGTTC Primer view: 1 TCGACTCTGAGGATCCGAGCACGCAGAGAGAGATCGGCATCGACGACGCCGCCATGGTAG 61 AACAGAGCGCAAGCGGAAGGCGGGGACCGGATTGGATCATACCCGCGGTGCGGAGGGCGA 121 TGCCGAGGCTGGAGAAGCTTCTTGGAGCTTCGCTTCTCAAGTTCTGGTAGCGGCGTAGCA 181 CAGGTTGTCTTGGCAGCACTTTTCCCTTGGCGAGGAGAGGAGACGAACGAGCTGATGTAT 241 ATACGCGGCGCCGTTGTGGTGGGGGTCGTCGCGAATCCTCTGGAAGATGTGGAAACCCTC 301 GGGTGCGGCAGCTCTGCGCTCCCAGAGTCGCGTATATGGGCCGTGAATTTCGTGGGCCGC 361 GGGCACGAGCGCGGATCATATCAGGTTGCATTGTTTGCTTCATCATCATTCGCGTATCTG 421 AACAAAATTGTGTCCTCTTGAGAAAAAATCTATACAAAATTGTGACAAGTAAAAGTAATA 481 TGAATGACAGTTATCCGCTCAAAAAAAATTTATTTCAGGACGGAGGGAGTGAATCAAGAA 541 TGTACAATTATTGCCGACCATTGGATAGTCAAAAATTGGCTAAGAATTTGTTAATTCATG 601 GTTGTTTAAAAAATTGGCAAAATTTGTGAAGAGAGATATGAGGAGGTTGGTAAGATTCTT                                         >>>>>>>>>>>>>>>>>>>>>  661 TTGGAACTAAATTTTTATATACCAACCAACCAAAAATTAAAATAATATCGGTTGCCAAAC 721 TTTTGATAAGGCAAATGTTTGCAAGAGCCAAACACACTGGAAATGTTTAGGAATTCAGAA                                                            <<< 781 CTGGAGAAAAAGAACCTCCTACTGCTTCGATAATATGGTTACCATATTCATCAAGCGTGG   <<<<<<<<<<<<<<<<<<                                           841 TACAAAACTGGAGGGGAAAATCAAGGGTGCGATTAATTTGTGGAGCTCCGTCAAGACTTA 901 TCTTGACA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0