Sequence ID: DB069A20_F1.ab1

Motif: (AAG)4 Motif length: 3 SSR: AAGAAGAAGAAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 626 20 55.16 40.00 6.00 2.00 AATATTGGACATGCGAAGAC 169 907 907 4.00 2.00   REVERSE 794 21 54.99 47.62 5.00 3.00 GATGCAGCCTGTGTATCTTAC Primer view: 1 GGATGCCGTCGGCTCTGTCGAGTTTGATTTGTCTTGTGGAAATTTGCTCGCAAATTTGAA 61 TTCTCATTGATATATCATTGATACCTCGTTGATATATCATTGATTTTGTCCCATGCCATT 121 TGAATTTCGAACATATTTCATTGATATCTCATCGATGTATCGTTGATTTTGTCCCGTACC 181 ATTCGAATATCATTGAATTTTGTTGCATATCATTGAACTCATTGAAATATCTTTGAATTC 241 AATGAAATTTATGTGTATATCATAGAAAGTTCTGTAGGCATAAAATCATTTCAATGATAT 301 GGCAATAAAAATAATGATATAGTAATTATCTCATTTTTAAATAAAAATAATAATTTATAT 361 TCAATTGATATGGTAATGAAATCAGATTGGTATTCTAGTAATGATGTTGAAAAATTCCAG 421 TGGTGTATTAATTGTATAATATACTCTAGTGAAATAGTTGCAAATGGTTTTGAAATAGTA 481 ATGATGTTGATTAGATGACAGAGCACGTGGGTCGGACCTATCCGTTGAATGCTGATAAGA 541 CACGAAATAGGGGCTGCTACACTTTATGACAGGCCAATGCCTGGTTGGAGGACCGGTTGC 601 TGGTAACTAGCCGGCCCGGTAAAAAAATATTGGACATGCGAAGACAGGTCAAAGAAGAAG                             >>>>>>>>>>>>>>>>>>>>               661 AAGAAAACGAACACAATAACTGTTGAAGAGCTACACAATGTACCACTGTTTGGGGAAAAA 721 ATTCCGTAAGCAATGACCTATGCATGCGAAGAGAGTCCTTACCAGGGGAGGCAGTAAGAT                                                        <<<<<<< 781 ACACAGGCTGCATCGCAGCGTGTGAAATTATTTGCTTAATTTGTGTTCAGTGCGTGTCCA   <<<<<<<<<<<<<<                                               841 CGTTCTAAGTGCAATTCTGACTATGGTTTGGCCTTGTCCTGCTCGACATCTTATGATCAC 901 CCAGGCA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0