Sequence ID: DB079L09_R1.ab1

Motif: (AAAG)3 Motif length: 4 SSR: AAAGAAAGAAAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 477 18 55.41 50.00 4.00 1.00 ATCGAGGCCCATACTGTT 182 907 907 3.00 0.00   REVERSE 658 22 54.89 40.91 3.00 0.00 CCTTTCTTTAGGGTAGTGATTG Primer view: 1 AGGATCCATGTCATGGAGCGCATGGGTGTGCTAACTAGCTTGGTTGGCCATGTGATCGAG 61 CCATATCGATCGATCCATGCACATTCAATCGTGCCAATGGGCATTTCATATATGGTGTTG 121 TTTTTAGCTTCAAACCCAGAGAGTTTAATTGTTACGCGAATCAAGATCGAGCATAAGTTT 181 CCTTCCTCGGAATAATATCTAGTTTTATTTTTTATTCTGGATTTGTCCTGTTTTTTCTTT 241 GGGGAATTTTGTTTTTGAGAAATTGGATCTCTGTGTCTTTCCTTAGGACATGGCTATTGG 301 GTCACAGCCCAAGAGTGCTGCTTACAAGATATAGAATCTCACAGGAGAGTGGCTTTTCAT 361 CGGCCCAGGAGGGGGACGACTCACACAATTAGGAATGAAATTTCTTTAGGAATTACTAGC 421 AGTAAAGGCCCATTTATCCCCCTAAAAAAGTAAGGCCCGTTTATTAATCGAAAGGAATCG                                                            >>> 481 AGGCCCATACTGTTGCCATCGATGTCAACATGATGCATGCAGTATTCTAAAAAAAAAAAC   >>>>>>>>>>>>>>>                                              541 ATGATGCATGCATTCTTTTTATTTTGCAGGTATGATGCATGCATTCTAATGAAGATCAAT 601 CACTGCCCGCTGTCAAAAAGAAAGAAAGAAAAAGATCAATCACTACCCTAAAGAAAGGTT                                       <<<<<<<<<<<<<<<<<<<<<<   661 CAAGCCTAAAAAAAGATCAAGTTAGTGACATACAAGGAAAATTAATTTAATGAGACATGT 721 ATATTTATAGCAGTATGTAGTCGTTTACTCTGTACAACGACGTGACCCAAGACCCTCGTT 781 ACAAGTAACAGACAAAATTAGTTTTTTTTACATCATTGATACAAGCTTGGAGGATACACA 841 AAACATATATATTTCAAACCTGATTTCGCTACTTATTTTGTGAAAGAAAGGAAACAAATT 901 ATGCTGT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0