Sequence ID: DB091I18_R1.ab1

Motif: (AAT)4 Motif length: 3 SSR: AATAATAATAAT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 552 21 54.92 42.86 3.00 0.00 CTTTCTTCCTCCTCAGTTTTC 153 906 906 4.00 2.00   REVERSE 704 21 55.57 47.62 5.00 3.00 GTAGCTGCTTTGCTATCACTG Primer view: 1 AGGATCCCTCACGTAGAACAGCCGAATAGGCGTTCCTCCAGCTGCATCGGTTGGTGGTTC 61 TTCAGGTATATCACGGTAAAGATGCTTGATAATGAAGAGAGCAGTATCATGGTCTCGCCA 121 ATAGTTCCTACAGGAACCAACACCACAGGCTTGTTAAAATGTGAAGCAGGCATGGAAAAA 181 ATAATTAGCAAACCAACAGGAATGATGATCGAGCTTACGTATGAGATCCCAGGGCAGATA 241 AATATGGATGTTGAAATGTCTTCTCCTGCAATTAGATTATATTTTAAGACTGTAAGCTCA 301 AAAATATTCATTCACTGCAAAAAATGTACACAAGAAAAGCTAACTAACTCAGCCAAAAAA 361 GGTAAAAAAAAGGGAGTGGAAGGTGGGCCATGCTCGCATTCAATTTACTGCCTATAATAA 421 GTAACAATACGCCAATACAGAGAGAAATTGCAATTTGATTCAATTGCAAGAGCTTAATAA 481 CCTGAAGCACATGATCAACCCGACCATCTGGTGAACCTGTCAACCTTTCCATCATCATGG 541 AACCATACGATCTTTCTTCCTCCTCAGTTTTCTCACCATCATCTAAATAATAATAATGAT               >>>>>>>>>>>>>>>>>>>>>                            601 CAGAATACTGCTTGTTTGCTGCTTGGAATGTGTAAAAGCTAATAAAAGCTTGATTTAACG 661 GTACCTTCCGTGTCATTTTTGCTCAGTGATAGCAAAGCAGCTACTGCTTTAACCTAAACA                          <<<<<<<<<<<<<<<<<<<<<                 721 AAACATACAAACCAACTCATTAAGCAAGAGCTATGCTAGCAGTTCTCCATCATTCACAAG 781 CGTCAAAGATTGATGTATCAAAGGAAACCTAGATCCACATAATCGCTTTCGAATTGTATG 841 AATTAAATGTAAATGCAACACATCAGCATTCCGGAATAAATAGCAACCTTCAATGACTTG 901 AACTGA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0