Sequence ID: DB060H07_F1.ab1

Motif: (AGGTTC)3 Motif length: 6 SSR: AGGTTCAGGTTCAGGTTC SSR length: 18 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 168 21 54.49 42.86 4.00 2.00 CCCTATGATGGCTACATTAGA 149 906 906 4.00 2.00   REVERSE 316 21 55.90 42.86 2.00 1.00 ATGCTATGAACCAAGAACCTC Primer view: 1 TAATCTATTTGTTGTTCCGAATAATATTGATATCTTGCTTCATCTATTTGTAATAGTGCT 61 TATTTCTATGAGTACTTGTATAAAGTTTCGGGATGACACTTGGACGAAGAACAGCTCGTC 121 CTGTTGTGCCCATTCAAAAGGCACATCCTATTGAGAATAATGTTGGTCCCTATGATGGCT                                                   >>>>>>>>>>>> 181 ACATTAGAGGTATTGTAGATACTCCACATGCTATATACATAACACTTAAGTGTGAGGTTC   >>>>>>>>>                                                    241 AGGTTCAGGTTCACGTTGAATTATAATTTCATTTTCAGTGTTCAGTCATTAGTTTGAGGT                                                          <<<<< 301 TCTTGGTTCATAGCATGCCGAACTGACTATTATTTCTTAGTTCAAGTTTTTAAGGTTTAG   <<<<<<<<<<<<<<<<                                             361 TCTGCAGACTAACTAAACCGAAACATCATCCGGATAAGAAGCTTTATTACTGCTGCTACT 421 GTTGTTACCGCAGTCAAAAACTAACACTAAATCATGACTTATACTTAGAATAAAAGATGA 481 CTGAAATTTACACAGAACCTGAACCTCATACTTAAAAGGCATTCTCATAGTTTCACTGAA 541 TCATTACCAAGGACAGACATAGCAATTTTAATTATTACAACCTGCAAGTTATTCAAAATC 601 TCCTCTGTGTCTCCATACTGAAACTAATACCAAGTCTAAATAGCCAAGGCTATATTGAAA 661 CTACAACAAGCTCATCTAGGAACTAGCTAGACACACACATACGAACTTGAAGGTAGATGA 721 AGACACACAGCCGGAATTGGTTTCTCCGGGACACACAAGCAACAAAAATCACTAGGTAAA 781 GCCTTTACCTATGGTTTTTTGTCCTTCACCTAGGGTATTTGTGTCTAGGGAAAACTCTAG 841 TTTCTAGTAGTGCCTTTTCGTTATTGAAAACAATAGAGTTGCCATTATGAGTCTTGATAT 901 GTACTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0