Sequence ID: DB070A19_R1.ab1

Motif: (AAG)4 Motif length: 3 SSR: AAGAAGAAGAAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 234 20 55.51 40.00 7.00 2.00 CGTTTGGTAACCATCATTTC 193 905 905 5.00 3.00   REVERSE 426 21 54.06 42.86 4.00 2.00 GCTAGATGGAGAAGAAGCATA Primer view: 1 TCGACTCTGAGGATCCAGGAATTATACACTCTCAAAAAGAATTTTAACACCTATCGCTCA 61 AAAAAAAGAATTTTAAAACCTAGACAACTTCTGTCAACCAAAACCTAACATCCCCTAGTT 121 AAAAAATGGTTTTGTTGTTGATGAGTCAACTGATTTTCAGTTAGAGATTAGTTGACGTGG 181 ACGTTAGAGCTTATCACGTGAGAGATGAGAGAGTGAGCATGACCCTTACAATCCGTTTGG                                                         >>>>>> 241 TAACCATCATTTCAACCTATAATTATAGAATTAATTTTGAAATTTCAGAAACAAATTTGT   >>>>>>>>>>>>>>                                               301 TTGATTGGCACGAAATTGGCCACATGAATTCAATTTCAAATTTTATGGAATCACGTTGTA 361 ACAGGAATTGGTCAAAAGAAGAAGAAGAACATGTTTTTATAAAAGTATGCTTCTTCTCCA                                                <<<<<<<<<<<<<<< 421 TCTAGCGCCTCCACGAATTTCACGGCAGAGTCGGCAGCAGAGCTACGGGAAGAACCGATG   <<<<<<                                                       481 CGGCATGCCGTCTCGAGGATGCAGCAGCGCTACAGGAAGCTGGCCATGGTGCCGGCTGGG 541 CTCGAGGAATCTCTCAAGAGGCTTCTCCTGGAATACCTTGCGGTCAATGCTCCAGGGGAA 601 ACTGATGCTCTCCTCCAGGTTTGTACCATCTCTGAAACTTTGTCACACAAGTGCCCGCCT 661 AGCAAATCTTTGAAGCCGGTGCCAAAGTTGAGGAGCCACATGTTGGAGATCATCCCAGCG 721 AAGTGTGAGATATTTGACTATCATCAGTGTCATTGTCAAAAGGGTTGAAGCTGCATTACT 781 TAGATACGCACTACAAGGAGCAAGTTCAACTCTCTGACCTGTTCTTTGCTCTTTTGTACT 841 CCTACTTTCATTGGCTCTGGTAGATGCCTTTTTCAGAATTTATCCTTCTTTATCTGATTA 901 TAGGG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0