Sequence ID: DB081A13_R2.ab1

Motif: (ATGCCG)3 Motif length: 6 SSR: ATGCCGATGCCGATGCCG SSR length: 18 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 29 19 55.00 42.11 3.00 2.00 CAACAAACAAATCCTGTGC 212 905 905 5.00 1.00   REVERSE 240 21 55.17 42.86 2.00 0.00 ATCTTGTCTTCCGTGTATGTG Primer view: 1 AGGATCCCGCTGCTCCCGATCGGAAACCCAACAAACAAATCCTGTGCGTGCGCGGCGCGC                                >>>>>>>>>>>>>>>>>>>             61 AGCATCCGAACAACCCGACCACGCATACCGAAACGAGCCGGGGACCGGTACGGCGAAGAA 121 GCGACAACGCTACCATGCCGATGCCGATGCCGGCGTGCCGAGAGCTCGCGGCGACGGCTC 181 GGACGGCGCGCGTGGGCTCAGCAGCTCGGCGGGTGGATACACATACACGGAAGACAAGAT                                          <<<<<<<<<<<<<<<<<<<<< 241 TGGATGGGAACACGCCAACGGGACCAACAGGAAAATGGGGGCCTCCTTTAACGGTTTCCG 301 AGGCGGCGGCACACGCTACGTACTGTCCCTGGAAAGGACCGGTCGGTCGATCAGGTGCGT 361 GCTAGCGGCCGGGCCCGAGTCTTCCGCCCCCGCATGGCATGGCTACGGACGGCGGCACCG 421 CGACGTGCCTGTCTTTGTCAGAGCAATTCGACAGCAACAAGGAGGACCCCATGTTTGGAA 481 CCAGACCTTTATTTTTTACGGAAAGCTTTAAGTCATAAGATAAAATTACAAGTAGATAAA 541 ATTACAAGAAAAGGTGTTATCTAAAGTTATTGAAAAGCATGGCAGTTCTTTCTCTTTGTT 601 CTATGGAAAACCAAATTAAGCTCTTCCTTAAATAGACTGCTGCATCTGTAAAGTGACGGG 661 GTAATCCCATTGAAGATGCAATCGTTGCGAATCCTCCAGATACTTCATGCTGCCGCAGTT 721 ATGATTTCCATGTGAAAAGTCACTGATGGCTGTAATATGCTCATGTACAAATTGTCCAGG 781 GGTGAAATTGGAGCAAATGTCTCTCCAGCAAGTCATGGCAAAAGGACGGTGGAAGAAGAG 841 ATGATCCCTGGTTTCAATAATGTTTCTGTTGCAAAGAACACATGAGTAAGACTGGAGGTG 901 GAAGT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0