Sequence ID: DH021M07_F1.ab1

Motif: (GAA)4 Motif length: 3 SSR: GAAGAAGAAGAA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 25 21 55.16 42.86 5.00 3.00 GTTCATTGATAGTGCCACAGT 171 905 905 3.00 2.00   REVERSE 195 21 55.33 52.38 5.00 2.00 GACCAACGGTCTAGGATACTC Primer view: 1 AGACCTTCTGGCGGCTAGCCATGGGTTCATTGATAGTGCCACAGTGTTGCATTAATTTGG                            >>>>>>>>>>>>>>>>>>>>>               61 CTCGACCATAGTTTTGAAGAAGAAGAAAAAAAAAGCACATTTGACAAGTCTGTCTTCAAA 121 TATAGTACGCTGAAACCCTGAACTTTTTGCTCATGTTTGTGGCTTGCACCGGTCGAGTAT                                                         <<<<<< 181 CCTAGACCGTTGGTCCTGAGCAACTTTGAACAGCCATCGCTCGTACATGAATATATATCT   <<<<<<<<<<<<<<<                                              241 CTCCTCGAGTCACAGGCTATAAGGTTAATGTTTAAAATGCGATCTCCTAGGTCCTGGGGA 301 GCTATCTAATGCGTCAACTGGCCAGTCAAAAGCTGGTTGCTTGATCAACGGAGCAAACTG 361 TAAGTTAAACACTACAATGAGCACCTTCTATGTGTGGCCCAACCAGATGGCGCCCTTTTC 421 TGAAATTTTTACAAGCTAGTGTTTCTCAACTTCTCAAGTGGATTCTGTTAAGTATATCAA 481 ATCTTCTCTTCTTATCAGATCGGTCTTTTAGGTGAAATGGCTAGATAGGGTATCTTAACA 541 TGGTATCAGAATCAAAGGTCTCGAGTTTGAGTCTCAATCCTCCCACATTTTCCCATCATT 601 ATCCACGTCGGGCGTGGGGGAGCTTTTTTCTTTCCACATCCACGTCGGATGTGAAAGTGG 661 GTGTTAAATATATCAAATCCCCTTTTCCTATCAGATCTTCATGCATATATACACTGTTGT 721 GACTTTTATTGTTTCTTGTCATTGCCATTTCGAAGAACTTATTTCACTGATAGTTAAGCG 781 CGCACGTTTAGAAATATAATCACGCATGCACAAGTCAACATGAAAAAGATGGTTTTTGAC 841 TGTGGCACTAGCATGTGAAAACAAAAATTGAATTTGGTGAAAATGACATGCATGGTGCAC 901 CATTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0