Sequence ID: DB070E17_R1.ab1

Motif: (TGTG)3 Motif length: 4 SSR: TGTGTGTGTGTG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 195 20 55.28 50.00 2.00 1.00 CTATGCTTGGCTTCTCTGTC 144 903 903 4.00 1.00   REVERSE 338 21 56.26 42.86 6.00 1.00 TAAAGCTTGAAGAACCTCCAC Primer view: 1 TCGACTCTGAGGATCCTTCGGTTGGTGGTTACAGCCCATTTCAGGGGCAAGTTCTGGATT 61 CCGTCGTCCATTGCGCGTATTCCCCAAGATTTCTTGGCGTCGGCATCTTCTTTTGTGGTG 121 GCCATGGATCGGTCTTCCCCAACATCAATTGCTCTGGCTACACCATTTTCCTCCGGATCT 181 GGCGGAGGCATGTGCTATGCTTGGCTTCTCTGTCCACCGGTGGCGGCCGGCGTCGGTGTT                  >>>>>>>>>>>>>>>>>>>>                          241 CGTCGACGAATCCTACGACGGTCAGTGGTGAAGCAAGCCTTCAGATGTGTGTGTGTGTTC 301 GTGGCATCTCAAGCAACGTGGAGGTTCTTCAAGCTTTATGTGATACACGTGTTTGTAATC                    <<<<<<<<<<<<<<<<<<<<<                       361 TTCCTTTTTACTTTTTATCCTTATGTCTTGTACTGCGATGTAACCTTTTGTGGAGCTTCT 421 TTGCCTCCACCTTGATAGTAATATATTGCAGGCAGGGTTCCTTTAAAAAAAAATACCACA 481 GTTCTATACCGTGACTCCTTTTGTAGAACTCGGTGTGGAGTCGTTGGTTGTCACCAGGAG 541 ACGTGGGACAAATAACTGCTCTGTACTGAAGACTGCGGTATAGTATAGCGAACTAATAGG 601 AATTTTGTGGGCGTGCAAGAGGCTAACGCTGCGAACACACTGGCTCATTGGGTGGCCCAG 661 CTGGCTGTTAGGAAGTTACCGAGTACCAGTGCAGTGCTAGTACGTTGATTGATCAATCAG 721 TGTGCTTGGTTTCTTTTTTCTGCCATCATGCATGCTTGGTTATTTCTTATTTGATCCTTC 781 AGAGAACATGCGTTGTGTTCCAAGATACTCTCTCCGATACTAAATTGTTGTCGAAATATT 841 ACATGTTAAATTGTTGTCGAAATATTACATGTATCTAGACGCTTTTTTAAATAGATACAT 901 CCA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0