Sequence ID: DB092D16_F1.ab1

Motif: (GCTA)3 Motif length: 4 SSR: GCTAGCTAGCTA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 509 20 54.92 50.00 3.00 2.00 GTCCACTCGTCAAGATTAGC 149 947 947 3.00 1.00   REVERSE 657 22 55.12 40.91 4.00 2.00 GTAGCTGTCCATCAATTAAGGT Primer view: 1 GGTATTCGCGGTTTTAAGATAACTTTGCACACAAAATGCTGTGTCCATCCCGTATGCAGA 61 GGCTGAGGGTTTTTTCGTGCATCGCTCGACTGCACTTTAAGACAATATAATTAATGCATC 121 AATTATTGAAAAAAATTTGCCAACCTAGCTAGCTAGCTAATTCATTAATTATACTCCACT 181 AATTAGCAGGTCAAGTACAGTAGCCCGATCTCTTCGATCACCCCATTAATCCTCAACAGT 241 GAACAGCTTTAAATCGCCTGTTAATTCGTCACGTCTTTGCATTCATCTCTGCTTTCTTTC 301 AATCTTTCATTCTTTACATCTTAGCAGGTGAGAACCGCGCGGGAACAACTATCCACTAAC 361 TTGCAATTAATTATAACGACGTTAATTAATCTGCGCCACACACTGATAAAGCTAGCTAGT 421 AGCTTGCAACACATGAAATGGATACTAAAAATAGCTATTAAAAGTAGCAGACCTATAGAG 481 ATCGTATATATGCACTCCCAGCTAGGACGTCCACTCGTCAAGATTAGCTTCACAGAATTG                                >>>>>>>>>>>>>>>>>>>>            541 ACTTCGAGACTTGGCTAGCTAGCTAGTAATATCGGTGAAGTATATATTCAGCTAATTTGA 601 CAGAGCCTTGCAGGTGAATTGTAGGCATGTCGATCACCTTAATTGATGGACAGCTACAAA                                      <<<<<<<<<<<<<<<<<<<<<<    661 TCCAAGCAACTGTTTGGGTCTCAACCCAAGCAAATTGAGTGACAAGACACGCCACACGTC 721 GCTAGCTAGCAGCTAGCTTAGGGTAAGCTACAGTTTGTTGCATCACCAGCTGCTAGCTAG 781 GATATGTCTTCCAGCTTTCGTTTTCAAACTTTAAATTGTGAACATTTCCCTATTAATCAG 841 CCTCTCTCTATGCCGTGTGTCGACCAGCTGATCCTTCCCAGCCATGAGATCTGAGCTCAT 901 CGCTCACACCACTTTAATTTCCAACTGTGCATGAGGCCTCTGCTGTA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0