Sequence ID: DB087A01_F1.ab1

Motif: (TCGA)3 Motif length: 4 SSR: TCGATCGATCGA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 51 20 55.33 40.00 4.00 2.00 ACGTTCCCAACTCCTTTTAT 112 903 903 3.00 0.00   REVERSE 162 20 55.14 40.00 6.00 0.00 TAATTAGGCTGCTTTGCTTC Primer view: 1 GGGCCGTCCGACGGAGATCGTGCGGCGCCCACCCCCGCTTCGTTGTGGCCACGTTCCCAA                                                      >>>>>>>>> 61 CTCCTTTTATTTTGTTTTGTCCCCTGCGTTCGTCCGTCCTACTCGTCCCAGGCTCCTGGC   >>>>>>>>>>>                                                  121 GTCGATCGATCGACCGCGAGAAGAAGCAAAGCAGCCTAATTAATCAGCAGCAGATCTGCT                         <<<<<<<<<<<<<<<<<<<<                   181 CCCCTGCACGTTCCCTGCGCAATTGTTCAACCGTGCCATCATCCGGGGCGTTTGCGACCT 241 GCCCGGGCTGCGACAGAGCAGGGTCTCCGAATTAATTCCCCACCCAAAGTATATCCAATA 301 ATTTTGGCTTCTTTTTCATCAGATGCGCCATGCATGGGCCGATGGCCGGTGGCTGTACTG 361 CAAGGCAGAGCTTCAAATTAAGGACCAGCTGATATCCAGCTAGCTACCCCGTGCATGCAT 421 GTGCTATTTGACGCACGACGTGAAGAGCTTTCCAAGTTTTGAGCTCATGTTTTTTTTTCT 481 GTCGAAGATGAGGTTTTACCTCCGCACTATATGCAACATAGTACTAACGTTAAGTACGGA 541 GTACATGTATATGCTCACGTAGTACCTCCAAATTCTATCCCTCGGATTTCTGATCTTTTG 601 TGCCAGGTCTATTTCATGAAGTTGGTTGTTCTCACAGTTAGATTGGTATTCTGACAGTTT 661 GTTCGCGAAGGAAAAGTTTGACAACTCACCGTGAGGTTTGTCCTCAGCCATAGTGCATTC 721 ATATTTAGAATGATCGACTCGTGCATTGGTTAGTCATGCTCATACAATTTTGGTTTTCTT 781 GATTTGAGACATGAAAATATATAAAGTTGCGGAGGAATGATTTAGAGATTCCGACTTCAC 841 TTTTTGAAAACAACTTTTAAAATTAATTATTCCATTGTTGTTGTTTGTTCAGTTCCCTTG 901 TGC Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

---

Primer3Batch v1.0