Sequence ID: DB156N22_F1.ab1

Motif: (CCAA)3 Motif length: 4 SSR: CCAACCAACCAA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 15 21 55.50 42.86 8.00 2.00 CTCGTGTTACAACAAGACGTT 123 901 901 4.00 1.00   REVERSE 137 21 55.19 52.38 8.00 3.00 CTCTGATAGCTGTCGAGTCAC Primer view: 1 TTCTGATCCATTCCCTCGTGTTACAACAAGACGTTTAAGTTATCCTGAAATTTAACTTTG                  >>>>>>>>>>>>>>>>>>>>>                         61 GTAGTATATAAGTGATTCTGACAAATAAGATGACACAGCCAACCAACCAAGCGCGGGTGA                                                           <<<< 121 CTCGACAGCTATCAGAGGCCGAAACCAAGATTTTCAAATTTTGTTTCAAGTTTGAGCTAG   <<<<<<<<<<<<<<<<<                                            181 CATTCTTAATTACATCGAGCATATAAAGTACTCTTTCGTTTCAAATTAACTGACGAATTT 241 TCATAAGAATCTATACATATCCACATCAGTTAAGTTAATTTAAACGGAGAGCATATGATT 301 AGTCCACGAATTAGCCCATCCACTCATCCCGTTGCTAGCTACTCCAGCTGTCATGCATGA 361 AAACTTAATTACCACTACAGTAGCTGTGACAGTACTACTGTATATGAGTACTCAGGTTTG 421 ATTTGGGCAGGTGGCCTGTTTCGAAAACGACCTGGCACACTATCCCATCGGACAAGTTAG 481 CACAGCTAGTACAATGACAGTAATGCTCGATATATGGTGTCTCGATGTGATGCTAATCCA 541 TCTTAGACCAGGTATATACTGTGTCGTCAGAGGCTAAAACCTTGATTACATTACCAGGCC 601 ACAGCTAGCTAGCCATGAGAACGACAGAGATCAGATGTTATAATTACCCATGGTTCACTA 661 GTCAAGTCAACCAAACATGGCGCGGGCATTGCTGCAAGAAAACAAAGATATCTATCTGAT 721 ACTAAATTTTTTTGATGTATCTATTCTTAAAAAACGTCTAAATACATGTAATATTTCGAT 781 AACAATTTAGAATCGGAGGGAGTACTACGTAAGTATCTCTAGTTCTGTATACAGTATGCA 841 AGACTTTTAATACTGTACAACTATACAAGCGCTAGCTAGCTCTACTGTTTCCACGTACGG 901 G Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0