Sequence ID: DB090H12_F1.ab1

Motif: (CATG)3 Motif length: 4 SSR: CATGCATGCATG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 710 21 54.96 47.62 3.00 0.00 GTAGTCTCACCTTTCCCTTGT 139 901 901 4.00 3.00   REVERSE 848 21 54.28 42.86 4.00 1.00 CTAAGTGCCAAGATTGAAGAC Primer view: 1 AATTGTTATTAGGTTTTCTTTACAGCATCTAGAATTTACCTTCTCTCTATCCGTACTGTA 61 ATTTTTGTACACGAAATTTGGATGATTCTCAGATTTAGGCATTTAGCCCGTGCGAAGTTT 121 TGCTGCATCCAAAAGTGGTCTTCTTTCTATTCGTACAGAATTCTTGTGCACAGATTTTTG 181 AATGATTCATAGATTTGGCACATGTGGAATTTTGTTGGTGTGATGTGCAAGATGTGCAGA 241 TTTCAATGACTGGCACAAATGTTAGAATGGAGTACGCCAGGGACGAAATGGTTGGCACAA 301 CAGTGATAGTTACCAAAAGAGATTAATCATGTACTGATTGTGTTCCTTTTCAATCCATGT 361 GTTCCCTCTCTCTAAATCCATTTTCTTCTCAATCCATGTGTTTTCTATCTCTCTCAATCC 421 ATGTGTTCTTGAGTTAGAAGGCCTGAATTTTGCCTTCGTTGCCCCATATGCCATCCATGT 481 CCTTGTTCTCTGCTAAGTTGAGCTCCAATAGACCAAAAAAAGGTGAAGTGGAGAGATCGT 541 CTTGGTGATATGGAGCACCCGATCCGATCAAGGACATACACAATGATTGTGGGGTCATAG 601 TACTAGGTACCGTCCTTTTGCATGCCATTACGTCAATGGCATTTTTCATTGCCGTCTACT 661 CCGCGTCAAATGATGGAGGGCTGGGATAGTTGGAGCAATAAGCTCGAGAGTAGTCTCACC                                                     >>>>>>>>>> 721 TTTCCCTTGTTACGGAGTACTAGATCGAAGAACTAACAGCTGTCATGCATGCATGAACAG   >>>>>>>>>>>                                                  781 CCCAAAGGGCTTTATATTCAAAGCAACCGAGTAGCACGACGTACGGAGTCTTCAATCTTG                                                  <<<<<<<<<<<<< 841 GCACTTAGCAAATGCCTTTGATCCACAACCCCTTTCAGTCTGCGGAGATGCCAAACTTAA   <<<<<<<<                                                     901 T Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0