Sequence ID: DB079C21_F1.ab1

Motif: (CAG)4 Motif length: 3 SSR: CAGCAGCAGCAG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 84 18 55.17 55.56 4.00 1.00 ACCACTCCAGTAGCAGCA 174 899 899 4.00 2.00   REVERSE 257 20 55.08 40.00 8.00 0.00 TTCTTTCCATGGATGTTCTC Primer view: 1 GCGCGGACCCACTTGCTGCTGCTGCTGCTGCTGCGATGCGAGGGACACCACGAGGCCTGG 61 CCAAGGACGGCGCTGCCACGTGCACCACTCCAGTAGCAGCAGTGCGCTCTTGGTCTCCCA                           >>>>>>>>>>>>>>>>>>                   121 GCAGCAGCAGCATCATCAGCCACACGGGGAGTGTGCCGGCCGGTGCCTCGGCCGCATCGC 181 CCCCAGATCGCTGGCCAGGCCTGGCCCTGGCCGGCCGCGTGCGCTGCGTCGAGTCCCGAG                                                            <<< 241 AACATCCATGGAAAGAACGAAATGCACTGCTCCCCGGAGAGGGCCCGAGCTAGGAGGCCA   <<<<<<<<<<<<<<<<<                                            301 CCGCAACGTTCCCCTTGCCTTTCCGCCACCGACGGCACGAGCGATGATGTCGTGTGCCGT 361 GGTGCACCGGAACGGGAGACCACACTACGTACTGGCCGGCCGGCAGGCCAATACTAGTAC 421 ATGCCATGTGCTCTTCTGTCTGTCTCGGTACCACCAGCCCGCATCGATCTATCATCGATC 481 CCCGGGTTGGGCACACAGTAAAACAGTCCCGTCATCTCAACACACAACCACCATGGGGGG 541 CCAAGGAGCTAGCGAAAGAGCAAAAGTGCTTGTTGGATGGCCTCCTCCCGCTGAACCGCG 601 CAGTACGGCAGCACGAGTGTGAGACTGAGTGGCTCCTCCATTGTCCATTCGGGGGAACAC 661 GACTTGGACAACATCGTTTCATTTTCTTTTCTTTTGGCGCACACGTTGGGTTGACTGACG 721 ACGGTTCCGGCGACCACGCCACGTACGCGTGCGACGACGGGCCGCTGGTTCGGTACACTG 781 AACCAGACGGAGTAGTGCCACTTCCTCTTCCTCCCCGAGTCCCTGATCGTGACACTATCC 841 CACACACACACACACACACGGGGCAGAGGGAGGCCTGCTTTTCATTTTCAGCTCACATA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0