Sequence ID: DB079C21_F1.ab1

Motif: (CACA)4 Motif length: 4 SSR: CACACACACACACACA SSR length: 16 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 774 21 55.86 47.62 3.00 2.00 TACACTGAACCAGACGGAGTA 123 899 899 3.00 0.00   REVERSE 896 20 55.24 40.00 4.00 2.00 GTGAGCTGAAAATGAAAAGC Primer view: 1 GCGCGGACCCACTTGCTGCTGCTGCTGCTGCTGCGATGCGAGGGACACCACGAGGCCTGG 61 CCAAGGACGGCGCTGCCACGTGCACCACTCCAGTAGCAGCAGTGCGCTCTTGGTCTCCCA 121 GCAGCAGCAGCATCATCAGCCACACGGGGAGTGTGCCGGCCGGTGCCTCGGCCGCATCGC 181 CCCCAGATCGCTGGCCAGGCCTGGCCCTGGCCGGCCGCGTGCGCTGCGTCGAGTCCCGAG 241 AACATCCATGGAAAGAACGAAATGCACTGCTCCCCGGAGAGGGCCCGAGCTAGGAGGCCA 301 CCGCAACGTTCCCCTTGCCTTTCCGCCACCGACGGCACGAGCGATGATGTCGTGTGCCGT 361 GGTGCACCGGAACGGGAGACCACACTACGTACTGGCCGGCCGGCAGGCCAATACTAGTAC 421 ATGCCATGTGCTCTTCTGTCTGTCTCGGTACCACCAGCCCGCATCGATCTATCATCGATC 481 CCCGGGTTGGGCACACAGTAAAACAGTCCCGTCATCTCAACACACAACCACCATGGGGGG 541 CCAAGGAGCTAGCGAAAGAGCAAAAGTGCTTGTTGGATGGCCTCCTCCCGCTGAACCGCG 601 CAGTACGGCAGCACGAGTGTGAGACTGAGTGGCTCCTCCATTGTCCATTCGGGGGAACAC 661 GACTTGGACAACATCGTTTCATTTTCTTTTCTTTTGGCGCACACGTTGGGTTGACTGACG 721 ACGGTTCCGGCGACCACGCCACGTACGCGTGCGACGACGGGCCGCTGGTTCGGTACACTG                                                         >>>>>> 781 AACCAGACGGAGTAGTGCCACTTCCTCTTCCTCCCCGAGTCCCTGATCGTGACACTATCC   >>>>>>>>>>>>>>>                                              841 CACACACACACACACACACGGGGCAGAGGGAGGCCTGCTTTTCATTTTCAGCTCACATA                                       <<<<<<<<<<<<<<<<<<<<     Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0