Sequence ID: DB092L23_F1.ab1

Motif: (TTTG)3 Motif length: 4 SSR: TTTGTTTGTTTG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 433 20 52.42 40.00 4.00 0.00 GAACCAGCTCAATTTAACAC 141 899 899 4.00 0.00   REVERSE 573 20 55.18 40.00 4.00 2.00 TTTGTACCCCATTTGATAGC Primer view: 1 GCCATGAGGACCGGGCTGGCCGGAACTCGAGTGGGTCGGGCCAGAGAGCCGGGTCGCGCG 61 CGATGCCCCACACATTGACCACCAGCTCCGTGCCTTTGGGGATGCGGTAGCCGGCTACGA 121 AGCACTCCTCGGAGGCCATCCGCGGGAGGGACAATGGCGTAGATGGGTGCGGGCGGAAGG 181 TCTCCTTGATGACGGCGGTGAGGAAGGGGAGGCGCGACATGTCGCTCTCGGAGACAAGCC 241 TGGCCCGGCCAACGACGGCGTCTAGCTCCTCCTGCGCCTGCTGAAGGAGGTCTGGATGCC 301 GGATCAGCTCCGACATGGCCCACTCCACAATGCTTGAGGTCGTGTCAGTGCCCGCCACAA 361 ATAGGTTCTGCACAGATAAACAATATAGTGATTACATATAAATTCTTGCATGTTACAGAA 421 GTTTTAAATTTTGAACCAGCTCAATTTAACACTTTGTGGATTTTTTTTTCTTATAGCGTA                >>>>>>>>>>>>>>>>>>>>                            481 CACCTTTTGTTTGTTTGAAAAAACAATATTACAGCTGAAAATGCTGAACATGGCTGTAAG 541 ACGGGGGAAATGAGCTATCAAATGGGGTACAAATTAAGGACTGGGCATGTGTGTCTAAAA                <<<<<<<<<<<<<<<<<<<<                            601 AATACGGAATTGCTTGGGCTCAGTCGCTTGAGATGGTCTTTTTCTCAGTTGACGTGTTTT 661 CCGCCGTCGGATGATCTTTGAGATTCGTGCGGGCGTAGAAGCAAGGATAGACAAAAGCAT 721 ATATATAGCCTAAAAATTGTATAAAATCAGGTATACTGGCTACCCGTTGTCTACTCGTTT 781 GGATGTAGAAGTTGCAAGTTCGAGTGCTATCTCCATGTTTTTTTCTCATTTCGTTTTTTT 841 CTTTTGTCTATGTTTCTTCTTCATGTTTTTTCTTTTCATTTCGTTTCTTTTTTTCTTTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0