Sequence ID: DB080K19_R1.ab1

Motif: (CTCT)3 Motif length: 4 SSR: CTCTCTCTCTCT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 675 21 55.25 42.86 6.00 0.00 CGATTAATAGAGGAGGGAGAA 152 898 898 4.00 3.00   REVERSE 826 22 54.91 40.91 6.00 2.00 AGTTTGCCCAGGTATACTTCTA Primer view: 1 AGGATCCACGCTGCTCTGGTACACGCCTGCAAAACATTCCATTACAGACAAGATACTGAA 61 GAATCAATTCCCCTCCTCGCTCTACAGCACCAAAAAACAAAAACAAAAAACTAACCATTG 121 TTAACAGAAACTAAAAAAGAAGAAGCAGAAAATTCATGTTGATTTTTACTCACCATCGAT 181 CTTGCTGAGCACCTTCTTGACCTTCTTGTTACAACCGTCGCAGTGGATGTTGACCTTGAA 241 CACGCATGTCTGCAAAGGGTTTGGGGATGAATTCACAGGGTGCAAGATGATGGGAATGGG 301 AGCAGCAAGCAAGAAGAAATGTCTGCAGCAAACGAAGAAGGAGAAGAAGAGTTGGAAGAA 361 CTGACCTGAATCTTGAGCACCTCCTCCTTGCTCATCACTGACTCCCTGCCTTCCTTTGCT 421 CCCACTGAGGACTGAGGCCTCGCTCGAACTCAGCCTCAGACTCAGATCAGGGCGTGGAGA 481 AGCAGAGGGCCAGCCACCATTGCTCCTGTCTCTTTCTCTCTTCTGTCTTCTCACACAAAC 541 CACAACGAAGAAGGAGAAACAGAAACGGGCTTTGGGTGTGGCACTCACGCAAAGAGCAAG 601 GGTGCTCGTGACTTGTGAGAGTGTGTTTGCTACTGCTGCGCTTGAAGAATGAAGAGAGCC 661 ATGATAAAGTACCGCGATTAATAGAGGAGGGAGAAAGAAGCCACCTCTCTCTCTCTCTAA                  >>>>>>>>>>>>>>>>>>>>>                         721 GATCAGCTCCTTGAAGGTTTCTTTGCTCTTTTGGGCTGCCTTTGGAAACGGAAGCCCTTC 781 TTTCCGGCCACCGAGTAACGTATCTAGAAGTATACCTGGGCAAACTCCGGGCTGGGCCGG                           <<<<<<<<<<<<<<<<<<<<<<               841 GATTTATAAAAGCGCGACAGTCAAACCTGAAGCTCGAGCTCGGCCTGGTCCGGCCCGA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0