Sequence ID: DB091M22_R1.ab1

Motif: (ATTA)3 Motif length: 4 SSR: ATTAATTAATTA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 462 22 55.16 40.91 4.00 2.00 CATTCTGTTTGAAGACGTACAG 147 898 898 4.00 1.00   REVERSE 608 21 55.04 42.86 4.00 1.00 CAATCTACATAGCCCAGTTTG Primer view: 1 GTCGACTCTGAGGATCCAAGTAATATTACGAACTTGGCCACTTGACTGCTCAAATAAACA 61 CACACAGAGAGGAGTTAAGTATCTGGGCACCAAAGATACGGATGGATCGGCACTGGCTTT 121 CTCTCTCCTACGTCAGTGGTCCCCCAGCTGCTGAGGTCATAGTGCTGTTAACGCGGAGAG 181 GGCTTGAAGATTCCTGCACCTGGTCAGCTTCCACGTTGCTTCGCCAATGCACGCCCAAAA 241 TAGAACCTCTGACTTCTCCCACAAAATCAACGAAACCCCACTAAATTTTTAATCCCTCTA 301 GAAACAGGCTCCGCTTTACCACGCAAGACGATAAGCCTTTTCCGTGAGCGCAAAGCGAGC 361 AGAGCATGTCAAGGACACAAAAACTGAATTGATTAACTTTCTCTCATCAGTCGAAGATCC 421 ACGTACGTACAGCTCTATTAATTTCCAGTAGCATATTAGTACATTCTGTTTGAAGACGTA                                             >>>>>>>>>>>>>>>>>> 481 CAGAAGAAAAGGCCAGATTAATTAATTAATTCATTATACTTTAAAGCATATTAGCTCAAT   >>>>                                                         541 GGATCGGACACGAATAACGGCGGAAGAAATGCTTGAAACGCACATGACAAACTGGGCTAT                                                  <<<<<<<<<<<<< 601 GTAGATTGTTGAAGATTACGGTATCAACTATCAACAGCAACATATGAATATATAAACAGT   <<<<<<<<                                                     661 TGAGAAAGAAGTGCAGACACATGTATCTATGGCTAAACGACAACTTGGGATTTTACAATA 721 TTTGTTCTTCCCGTAGTAGCTTATAGTTTGTACAGATATATAAATAGATCCATTGGATCG 781 ATGATTGCAGGAGCAGCTGCTAATGTGGTTCCTGTTCAGGCATGCAAAGGGGCAAGGTTG 841 AATTGGTCAGCCAGCATCAGGGGCAACTGACCTTAAAAATTCCCCATCTCTTTGCCCC Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0