Sequence ID: DB086I03_R1.ab1

Motif: (TGTG)3 Motif length: 4 SSR: TGTGTGTGTGTG SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 528 21 55.44 42.86 6.00 2.00 CCATTGTCTAGTGCTAATGGA 157 933 933 5.00 0.00   REVERSE 684 21 54.70 42.86 6.00 2.00 GTTGAGCTAACAAGCTGAAAG Primer view: 1 AGGATCCCGAGCGAGATATCGGGGACGCCTCCATATGGCGATCAGGACAGGGCTTCTCCT 61 GGAGAGCGTTTTTGAGCCCAATTCATACAAATATAGCTATTGGGGGCCTGATGGGGCTTC 121 TCCTGGAGTTGCTCTAATTCCACGTTATCCTCTTCCTTTCCCATTCCACCGCATTTTTTT 181 TTAGGGGAAGCCTTTGTTCGGTTGTTCCCCGCTAAGCCTGGCGAACGACGGCCGGGCCGG 241 GTACTAGCTGGCGATGGGCCAGCACGGAACGGGTGGCACAAAATGAAATCGTGCCGTGTC 301 AGTTTGGCCCACGGGCCTGATTTAGGAAAAAGGCCCGGCACACTGAACTGAGGTGGTTAT 361 GGTTTTGACCACACAAGCTTCTGGCTTTGGCATCCCATGCGATTGCGACTCCGGGCAACT 421 GGAGAGACGGCGGCTACTTTTCATCTGACTTCGTCCTCCGCTCGCCTAATCCATCCTGGC 481 TCCGTTGTCCGCTCGCCCAATCGCCCACTCCATCTCAGTCGCGGTCTCCATTGTCTAGTG                                                   >>>>>>>>>>>> 541 CTAATGGATAAACCGCTTGTGTGTGTGTGCGTGCCCGCAGTGTGCTAGCGATCTGGCATG   >>>>>>>>>                                                    601 TAATTCTTAGGAGCGCTCGAATGCTTAAGAGGTTAACGAACTGAGTCGAACATCAGTTTT 661 AGGCTTTCAGCTTGTTAGCTCAACGAGCATGAGCTTAATGAGTCAAGCATCTCGTGACGG      <<<<<<<<<<<<<<<<<<<<<                                     721 CGAGGCAAAGATGTTTCAGGCCAGGCTGCCCTTGCCCATTGGCAACGTATATCTATATTG 781 CACTATCTGTTTTTATTTATTTTTGACGACCTACTAAACAAAACCTCTGCTCATTGAAAA 841 ATGGAAATGGATCCTGCTTCACGTACGATCTTTTGCATCCGATTTCATCAGGCGGATCTA 901 TCAAGTCCATTCCAACTATGCAGCCCTGACCAA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0