Sequence ID: DH019O05_R1.ab1

Motif: (ACAT)4 Motif length: 4 SSR: ACATACATACATACAT SSR length: 16 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 140 20 54.55 40.00 2.00 0.00 CAAACACATTCATCCACATC 131 896 896 5.00 2.00   REVERSE 270 21 55.25 47.62 6.00 3.00 GGTTAGTGTTAGACACGGTGA Primer view: 1 CAATTGTGTCGGCAGGAGTCTATGCCATCCGGCTAAAAACACAATACATGCAGTAGTTTA 61 CATACGTATGTGCTAATCCGGCCAAGATACAACTTCAAATGTATGCAGTACGACATCACA 121 GCATCCTTCGTGACATGCGCAAACACATTCATCCACATCCCGCATTGCAGCAATGCTAAT                       >>>>>>>>>>>>>>>>>>>>                     181 AAAAATAATTATATTCCATCAGACGAATCCACATACATACATACATCAGATGTGTTTTGC 241 CCCAAGCAATCACCGTGTCTAACACTAACCGGGATAAATTAAAAAAGAGACGTTTCATAA            <<<<<<<<<<<<<<<<<<<<<                               301 AAATCAAGTCCCAAATTTCTTCGTTGCATAAGCCTGCATGTATGTAATAGATGCACAAAA 361 TACGCACCACGTACGGTTCATTAAAATAAATGAACATGAAGCCACCACATTCGTCCACAT 421 AGGCTAAATAATCTACACTGTCAATCGCCGTTGGTCTAGATTATATCGGCTCGCATATAA 481 TGATAGAAACATAGAAAATACAAAACATTTTATTCTGATAGAAGTATACATACATATAAG 541 AGTGAGTCCAACATTGCTACCTTAGAAATAGAATCCAACAGAAAGATAAAGAGAGACAAA 601 ATAAAAAGAATAATTTATTTCAACTGAAACCACTTCATGTTATTCTTTTTTCCTAAAGTG 661 GACGCTAATCAGTCGGCATAAAACAAGAGGAAAAAGACCATCATGTTCATCTGTGTAGGC 721 TAAATTATGTCGATACTTGATCATCATGGGTTAATTATCAGAGGAAATATACTGTCAAAA 781 TCTTTTAACAAATCTAGCAGCGCAAATGCGCGGGTCACCGCCCTACTAGTTCTATATAAA 841 TACTAAAGCGTTAACTCCTTGTTCCATATTCATAGAGGTAGAATTCTGAGCCGATG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0