Sequence ID: DB094F22_F1.ab1

Motif: (GCT)4 Motif length: 3 SSR: GCTGCTGCTGCT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 92 21 55.09 42.86 4.00 1.00 AAGGTGAACTCTTCCTTCAAC 149 896 896 4.00 2.00   REVERSE 240 21 54.33 42.86 5.00 1.00 AAGTCTTCTGGCTCAGTGTAA Primer view: 1 GGCGTCTCGAGGAAGAGAAGAAGCCGTGGTGGCCGAGCTCTTCGCTGGACAGAAGAAGCG 61 GCTTGGGAAGATCGCCAAGGAGGCTAGGGCCAAGGTGAACTCTTCCTTCAACAACATGAT                                   >>>>>>>>>>>>>>>>>>>>>        121 CGGTGCTTCATCTGGGACTACTCGTGGCCGTGGCCGCGGCCGTGGCCGTGGAAGATCACG 181 CATTTCCCCGGCGCAGGCACGGGCTGCTGCTGCTGTCAGTTACACTGAGCCAGAAGACTT                                          <<<<<<<<<<<<<<<<<<<<< 241 GCGGGGCGCCCCGAAGAGCATCACGCTTCTAGTGGGCAGATCAAGCATCTGCTTCCTGCG 301 ACGTGCTGGGAAGGCGCCAGTGGTGGAGCTGATGGATTCAGAGGAGGAACGAGAGGGACT 361 TAGGGAAGAGGAAGAGAATATCCAAGCTATGGAAGCAAGACGCGAGAGGATGAGATCACC 421 GGCTGATGATGACGGTCCATCCACGCCGGTGCCGTCAAATGACGACACAAGCTCAGGTGC 481 AACTTAAATTTACTAATGAGCTATTATAAGATTAAGGAGTTAATTTTACATGTTCACAGC 541 AACTTGCCTTATATGTACCTTTTCAAGATTCAGATTCGAGTGGATTCTGGAAATACTAGC 601 TAGGCTCAGCGACATGGCGTGGATTCGGTGAGTACAATTTGACTATTAGAGCTCATCTCA 661 AATTTATGCTTATGATCTCATGGTAGTTATTTGCTAGCTACTTCTTGCGACTACTTATGC 721 TGCATTAAAATATTTTGGTTTGGCATAATGTTTTTTTATTTAATATTACATTCTTTCTAA 781 CTCGACCATATTACATACTTTTTTTATCCGTTCTCTCGGCATCCGTCAATGTTAAGCCTA 841 GCCTGTTTTATAATATTTTTATGAAATATTTGGGACACACAAGATATTAGTATCTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0