Sequence ID: DB091D08_F1.ab1

Motif: (CCTTC)3 Motif length: 5 SSR: CCTTCCCTTCCCTTC SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 155 21 54.42 42.86 4.00 0.00 CACCTTTCCTTAACTCTTCCT 156 895 895 4.00 2.00   REVERSE 310 21 54.95 42.86 3.00 0.00 AAGATGTGACTGCCATAGAGA Primer view: 1 TTGTCTGTCACCTCAGGTTCATATCCTTCATCAGCAAGATCTAGAATTGGGGTTTCAGCT 61 TTATATGGCGGCGCTGAACTAAAGCTCCTATTGTCCTTGGCAACAGGTCGCTTAGAAGAA 121 TTTTCAAGTTCTTCAAGTTGCTTCTCGAGAATGCCACCTTTCCTTAACTCTTCCTCGGCC                                      >>>>>>>>>>>>>>>>>>>>>     181 TCATCCACCTTCCCTTCCCTTCTCAAGGCTAGGGCCTTCTTTTTCAGCGCTAATAGTTCC 241 CTCTGAATTGCCAGCTTACTTTTTGGTGCTGACACAGCACAGGCGTTAATCTCTATGGCA                                                    <<<<<<<<<<< 301 GTCACATCTTCAGTGTGTGCATTTTTCTGTCCTTCTGGAGAGAGGACCTTTGAGTCAGGT   <<<<<<<<<<                                                   361 TGTTCAGTTTCCAAATCCTTCTCAAGTAGCTTTGCCTTTTTAAGCAATGACATGGCCTCT 421 GCAACATTACCAGCCTTCTTGTGAGCAACAGCCTCCCTTTTTAAAGCAAGCACTTGATCC 481 TTTAGTGCCTCTTGATTCACAGAATAAAGAGGTCCGTGACTGTCCATTTGTTTGTCATCC 541 TCTTCACTCCAGCCAAATGATTTTAGGGCAGCAGCCATATCTGGGTCATTGATGTCATCA 601 TCTGTAACATCAAAGTTGCTATCAAAGGCAAGATCATCAGAGCCACCAAGAATTTGTTCA 661 AAACTGAAAGCAGGGAAACTCGAATCGTCCAATAGTATGTCATCCTGGTTATCATCATCC 721 ATGTTCTGAATAATTGCAGCCAAATCATCATCAGACTCTTCCGCTTCACCCAGAATCTCC 781 TGTTCTTCTAGTTGCCCTTTCCAAATTTTAGCCTTCTTTAGTTCTTCTTTTGCTTCAGCA 841 AGTCTACCTTCTCGCTTCAGTACTAGGGCTTGTCTCTTCAGAGCATTAACCTGAG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0