Sequence ID: DB090B04_F1.ab1

Motif: (GGAA)3 Motif length: 4 SSR: GGAAGGAAGGAA SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 91 21 55.11 42.86 5.00 3.00 GATTTATAGCGTCCTGGAGAT 168 894 894 5.00 1.00   REVERSE 258 20 54.22 45.00 4.00 2.00 CAGCACAATAAACACGTACC Primer view: 1 CTGCTAGTGCTAGCGCCTTCTTGGTTACTCTAATTTGTCCCCCTGGGTTCCTTTCTTGGA 61 CTGTGGAGTCGTGGGGACGCACTCTGATGCGATTTATAGCGTCCTGGAGATGGTAAAGGC                                  >>>>>>>>>>>>>>>>>>>>>         121 CAAGGGAGGAGGTGTCACAGAGTTCTTGAGTGACGTTTGGGGAAGGAAGGAAGATAAGCC 181 CGGGAGGAGTCATGGAGACGAGGGAGACGGAACTAGCTAGTGTGCGTGCCCCGTGCCAGG                                                             << 241 TACGTGTTTATTGTGCTGGGCGGGGAGGACGGGTGTAGCACGGCACGGCGTGGCTCGAGA   <<<<<<<<<<<<<<<<<<                                           301 TAAGGCCGGTATTGGCGTTTTCTCCATCAATACCCCAACGGACAACGCCTCGCTCAAACC 361 TGGGGGCTGCTGCTCCAACGGTTTTAGGCTTTCAGTTTCAGGGGGCCTAGCTATGCGCCT 421 ATACCATGCCGCATTGCCGCCCCAGGGGCGCCGCGTAACGGCGCAAGGCAACGAAGAATT 481 AATCCCATGCCAGTCTCCGATCCCTCTTCTGAACTTGCTTTTGGTCGCTGGTCACGAATC 541 TCAGATACGGAGTATGTCGCTTTTGCCATTTTTAATTTCGGCTCTTGTTAACCACAGAAC 601 CAGTAACCACGCGTCAGGCAATGGTATCAGGCCCGTAGATGGGTATCAGCAGGATGCACG 661 ACCGCTCGGGGCCAACAAAATTGTGGGGCCCCAAACTCTGGCATGTAGTCATGCTATTAA 721 TCAAGAAAAAAAACAAATGACTTTGCTAATCATGCACGGGAAGGCCACCGGGCCACTCTT 781 GACTGCCTCTGCACGGGAAATCGATGTCTCTCCATTGCCTCGCTTGACTCGCCCACGGCC 841 TGCCTCCACGGGTCCTCGCCTCCTCCGATCAGCCCCTGCTGGCCTGCTCCCTGC Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0