Sequence ID: DB078M04_R1.ab1

Motif: (TTC)5 Motif length: 3 SSR: TTCTTCTTCTTCTTC SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 182 21 54.85 42.86 3.00 2.00 CTTCTTGTTCTGCCATCTCTA 148 893 893 5.00 1.00   REVERSE 329 20 55.01 40.00 6.00 0.00 GCCTCTGTTAAGAAAAGCAA Primer view: 1 CATTGCCATACAAAAATTTGAACCTTCATTACATTTCGGTAATAGGCGCTAAACTACTAT 61 TACTACTCGTCCTCATCAGTTTTGGATTTCTTCTTCTTTTTCTTCTTCCCTTCAGTGCCA 121 CCAGTCTTAGGCTCAGCATCCTCAGAAACATCCCGGTTCTTCTTTTTCTTCTTGGGGGTC 181 TCTTCTTGTTCTGCCATCTCTACATCCTCCAGCTTGTGCTTCTTTTTCTTCTTCTTCTTC     >>>>>>>>>>>>>>>>>>>>>                                      241 TCTGTTTCAGGTTCAGCTTCTGCAGCGCCATTGTCAACATCCATTGCCTCAGTTTTAGAC 301 TTCTTTTTCTTGCTTTTCTTAACAGAGGCATCACCATTCTCTTTGTCTGCAAAAGAATTT            <<<<<<<<<<<<<<<<<<<<                                361 TGGGGTACACATTGGAAGTACAAATTAATGCGGACTGACAAAATATGCCACGACAACAGG 421 TGATTTTACTTGAACTAATGAAATAAGAGATGCATCCTTACCGTCACCATTCTCTGAGAC 481 TGCGCTTGTCATGCCCTCAATAGCAGCTTTCATCACATCAAGGTTCTTACGTGGTGCAAC 541 ACCCTTGTCATAAAAGTCTAGTCTCTCCTCAACTTGTTCGCGCAGCTTCTGGCCAAAAAT 601 GGAGCTGCTCAAATCTGCAATTCAGAACCCAGTGAGAAAACAATCATAAAAAGGGAGTCA 661 GAATGTGTAACATTTGCAAACTAAATCCTTCAACTAACCAGAATAGCAGTCAATGCGAGA 721 GGCAATGGAACATTTGTTCGCCAGGTATCGAGCCATTCTTCCCTTGTTCTTAGTTGATGC 781 ACGGCCAATAAAAGATGAATGGAATATGAGGCCATACTTTGGTGTGTTTCCACGTGTTTT 841 AAGAGCCCTGTAATAGAATTAGGAAATCAATCATGAAACATCAGAAGCATGAA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0