Sequence ID: DB079H19_R1.ab1

Motif: (GTTT)3 Motif length: 4 SSR: GTTTGTTTGTTT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 111 21 53.88 47.62 6.00 1.00 AGTGTGCCTAGGTCATGTAGT 151 892 892 5.00 3.00   REVERSE 261 21 54.78 47.62 8.00 2.00 ACTCTTAAGGGTACCATCACC Primer view: 1 TCGACTCTGAGGATCCAACTTCAAGCCTGCCATGCTCCTCAGCATCTATACTTTGGGTCC 61 CCCAGATGTGATTTTTTCTAGGGTAGTTCTCCCTACTCCTATTTGACTGAAGTGTGCCTA                                                      >>>>>>>>> 121 GGTCATGTAGTCCTTGTCTAGTATTCCAGTTGTTTGTATAAGGTCTCGATAGTTTGGTTT   >>>>>>>>>>>>                                                 181 GTTTGTTTTTTGTACATATCGCATTGGCGGTGTTGTAGTAGCATACAGAATTTGAGAAGG                                                              < 241 GGTGATGGTACCCTTAAGAGTTTACAGCTTGTATTCAAAATATAGAATAAGGTATTCTTT   <<<<<<<<<<<<<<<<<<<<                                         301 GTGTAATTAACTCTTTGTAACTCTTATAACCGCCGCGGAATATATTGGCTTGGTTTGGTG 361 TGTGATGTAAGTGATGTAATGGACTAATGGACACATGCGCTTGAATGAACAATATGTTTC 421 TTCGGCTGGTTTGGTACTTATCTTTGAGGGCTCTTTGGTCTGCCTGAATCTTACAGGAAT 481 TTTACAGGTAAAGTCGCCCACATTTATCAAGGAAAAACAAGATCCAGGTATCCCAAACCT 541 GAAGGCAAATTCTGCTTTGGGAACCAGATTATATGTTCGTTTGCATGAACACAGAAGTGA 601 AGGACATTGTAAATTTCTTCAAAACCACATTTCTGTTTCAAAAAAACATTGAGTTTTCTG 661 CTTCATTATTTCAGAAATCTACCCTGTTCAAGAACTATGGACAGCATCTATTCGTCCTGT 721 TGAAATGCTAAGTCCTGAAACCATGATACAAATTATATGTCGTTTTAAAAGCATAAACTT 781 GATTGTTTGTCAAGAGAAGTTCGGTGTGAATAAGTGTCCTCATCTTTTTTTATAGAAACG 841 CATAAGGAAAGCAAGTATTTTTTTTGAGACTAGGAAAGCAAATAAAAATAGT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0