Sequence ID: DH031N02_F1.ab1

Motif: (TGCA)5 Motif length: 4 SSR: TGCATGCATGCATGCATGCA SSR length: 20 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 307 21 55.11 42.86 4.00 2.00 GGGATTAGCTGTTGTAAAGGT 133 891 891 4.00 3.00   REVERSE 439 21 56.53 42.86 2.00 2.00 CAGTTTCTTCATTTCCTACGG Primer view: 1 CTTTTCCAGCCCCTGCCTCGTCAACAACATCATGTGCCAAAATTTCATTCAATTCTGCAA 61 TGAGTTTTTGGACCTGGAAAAGACACATAATGATATCAGCAACGCAATCCATGACAGCAC 121 AAAACTGAAGAAGGCATAAAGATTGGCAAACCTCTGGGATGAAATCAATGAGGCCCTCTG 181 GGATAAGGATAACACCGTAGTTGTAACCAAGTTCTGCACGCTTGCAGATAATATCAGTAA 241 TATAGTCTGTGACATTCTTTAGTGTTTCCTTCTTCTCAGCGACCTGTATTAATGCATTCA 301 AAGTACGGGATTAGCTGTTGTAAAGGTACACAAGTTTGCATGCATGCATGCATGCAACCT          >>>>>>>>>>>>>>>>>>>>>                                 361 ATAGTTCCCGACAATTTTATCAAAAATATGTGAGCTAACATATAAGAATTAAGAAGTTCC                                                             << 421 GTAGGAAATGAAGAAACTGAAATGTGCAACATAAGAAAAGAAAATATGGGTAAAGTGCAC   <<<<<<<<<<<<<<<<<<<                                          481 AGAATTTCTTTTTTCATCCTGTGTACCATTATCTCGCTAATCTAACCAGAAAAATGGTGG 541 AATGAGATAATATAAACTGAACATAGACTTATAATGAACTTTATATATAAAGAGTTTCAA 601 ACCTCCTCGCCGATGAGTGCAACATTAGGGTGTGTTTGCAGAGCACACTCTAAAGTAATG 661 TGGGAAGCAGCACGGCCCATAAGCCTCACAACTGCATGGCCATGGCAAATATATATTAGC 721 AGGTGAACAAACGAAATGATAAAACATGTTAAAATGGATCATCGATTAAACATGTTAAAA 781 ACAGATCATCTATCATTCATGTACCAAGGATGATAAATGTTTGTAGTATGCCATACAACA 841 CTATGAGAATGTTGGCATCAAAGACACTACTTAGAATAAAAGATAGGATCA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0