Sequence ID: DB070O21_F1.ab1

Motif: (TATC)3 Motif length: 4 SSR: TATCTATCTATC SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 11 21 54.92 42.86 3.00 0.00 GGAGTTGACACAAAGTGAAAG 141 891 891 3.00 0.00   REVERSE 151 21 55.83 42.86 3.00 3.00 CAAAATAAGGGGACAGACAGT Primer view: 1 TCCCTCCCAAGGAGTTGACACAAAGTGAAAGGTGTTCTATATATCTATCTATCCATCCAC              >>>>>>>>>>>>>>>>>>>>>                             61 CAGGAATATTATGTATGCATCCACAGCCTCTACATACACTGCGCTTTCTTTTGAAACGAA 121 CAGAGCCTAAACTGTCTGTCCCCTTATTTTGACCAGGCCATCCACATCTTTGACTTGTTG             <<<<<<<<<<<<<<<<<<<<<                              181 TCTAGTCCACTTTGAACTTATAGTCTCCCGAACATGTTACTGCAGGGAACTAATTATTAA 241 TCCACTTAAACCGGCAGGAATTGTTACCGTGCTTCAATACATAATCGGATGCTTCTACTG 301 ACTGATTTTTTTCAGTGGCAATTGATATTATGCTATACTTGTATTGTTGCCTTGTTGGCA 361 GTCATGTATGAACGTAGTAGGGCTGAACATGTATGCATTCAAGTTTTGAACCGGAACCTT 421 GGCGCCCGATTCGAGAAGAGGGCTGGGAAAGCAAAAGAAAAGCGCATGCATATATGTGCG 481 GCTTTGTGCTTAAAAGGCAGAGATCGATCGATCGATGATCAGAGGAGTACATGTTCGTCT 541 ATATATAAAACAAAAAGAGAAAAATAAAGGAAGAGATCGAGCAGAGGGAGGAGCAGGAAC 601 TTTGGGCATGCATGCATCGGTCTGGTCTGGTGCTGCACTTTGGTCTTTGGTCTTTGGTGC 661 CGAAAGAGAAAGAGAAAGACCTCTCCTGGTATCGATCCCTGATGGATGGATGGATGCGAT 721 TCTCTTCTCCTTTCCTTTTTTCTCTTTTGGCAGAGATATAGTGTAGCACCTCTCTCCTGG 781 TATCCATCCGATTAAAGCCGGCTAAAGAGGTTGATCATGATGAGAATAGAAACAAAACTA 841 AAATGTGTACTGATGTCACACGCTTCTTCCGTTGATGCTCTGGATCGATCG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0