Sequence ID: DB068O19_R1.ab1

Motif: (GCA)5 Motif length: 3 SSR: GCAGCAGCAGCAGCA SSR length: 15 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 15 21 55.41 42.86 4.00 0.00 CCGAGGGAGTAATAATAATGG 143 932 932 3.00 2.00   REVERSE 157 21 54.92 42.86 6.00 3.00 TGGAGAGGTCAGTTGATCTTA Primer view: 1 TCGACTCTGAGGATCCGAGGGAGTAATAATAATGGCCTGTTCTAAAAAAACTGATGCAGC                  >>>>>>>>>>>>>>>>>>>>>                         61 AGCAGCAGCACTGGTACATGCCTTTTGTAGCAGTCTGAAGTAATCATGAACAACTCAGAA 121 CCTGCCTTCTACAGAATAAGATCAACTGACCTCTCCAGCACCGAAGAACAAGAATGTGTG                   <<<<<<<<<<<<<<<<<<<<<                        181 ATCAGCTAAAGTTCCACCAACAAACTTCAGACCAGCAATGAGGCCTGCAAGAACCACCGC 241 AGCCGTTCCCTGTGGATACCACGTTTTCCAAAAGTTAACTTCTAACAAGTAGCGCGATGC 301 AACACATCAACAAATTCACTGGTTTTTGCTGAGAGAGATCATAATAGATCCAAGATACAG 361 AAACAAAAATGAAATCTGGTCCACTACCTGAATATCATCATTGAAGGCAAGATGGGTTGG 421 CCTGTACTTCTCAAGAAGGGTAAATGCATTATGATTAGCGAAGTCCTCAAACTGTAAAAA 481 GTTGAAGGAGTTTTGTTTTAAGTGTCCATGATCGATAACAGCTCAATTGCAAAGCAAAAT 541 AAAGATTTTTCCTACTGTTGACTTCTCTAACCTGGACAAGAACCTTCTGCCCATAGTTCT 601 GCTTAACGGCAGCCATGAACTCATCCAGAAGTTCAGCGTATTCCTAAATCAAGCAAGAAA 661 TGGAGACGAAATGTGAGAACTCATGACCATACTGTGAGAACTGAATGGGCATAGCCAAGG 721 CATCAGAAGTTGGAACCTGGCCAGTGGCTCTTTTTTGCCGTAACCCAATGTAGAACTCAT 781 CATTCAGTAACTCTTCATTGTTTGTTCCCACATCAATGGTGATAGGTAAGCACTGGGACG 841 CAAAACGGAAATATAGTTGCATAAAATTTATCAAGATGCCCATTGCAAACAGAACAGCAC 901 CAGATGTTGAGTACTTACAGCAGATGGCCTGA Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0